Recombinant
RabMAb

Recombinant Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573)

Overview

  • Product name

    Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)]
    See all GABA A Receptor alpha 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5401(2)] to GABA A Receptor alpha 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GABA A Receptor alpha 1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain, Human lung and Human cerebellum lysates IHC-P: Human brain, colon, kidney, Ovarian carcinoma, spleen, cerebrum and glioma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/1000.

For unpurified use 1/50 - 1/100

See IHC antigen retrieval protocols.

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Involvement in disease

    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling GABA A Receptor alpha 1 with Purified ab151573 at 1:1000 dilution (0.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 0.7 µg/ml (purified)

    Lane 1 : Human brain lysates
    Lane 2 : Human lung lysates - negative control

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 52 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    According to the paper (PMID: 29467616), brain is positive and lung is negative tissue

  • All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR5401(2)] (ab151573) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 52 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling GABA A Receptor alpha 1 with unpurified ab151573 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151573 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151573 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using unpurified ab151573 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab151573 showing -ve staining.

References

This product has been referenced in:

  • Tabassum S  et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). Read more (PubMed: 30872728) »
See 1 Publication for this product

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