Overview

  • Product name
    Anti-GABA A Receptor alpha 1 antibody [N95/35]
    See all GABA A Receptor alpha 1 primary antibodies
  • Description
    Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
  • Host species
    Mouse
  • Specificity
    ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Fusion protein corresponding to Mouse GABA A Receptor alpha 1 aa 355-394.
    Sequence:

    KVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATI


    Database link: P62812

  • Positive control
    • WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.
  • General notes

    The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Involvement in disease
    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all

Images

  • Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.

    Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

  • Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 52 kDa



    Block: 1% BSA.

    Primary antibody incubated for 2 hours at RT.

    Secondary antibody incubated for 1 hour at RT.

References

This product has been referenced in:
  • Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . Read more (PubMed: 25987849) »
  • Tan S  et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . Read more (PubMed: 21712993) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

To confirm with you, four antibodies are mentioned in your email. However, 2 copies of the questionnaire for ab124025 have been provided and there is no questionnaire for ab11259.

I can provide a response for ab94585,ab124025and ab31928. If you require further help regarding ab11529, please do not hesitate to send the questionnaire and I will be pleased to help.

I would like to reassure you that ab94585 and ab31928 are tested and covered by our 6 month guarantee for use in IHC-Fr and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement. Regarding ab124025, this is tested and guaranteed in mouse, but has not been tested in frozen sections. Could you confirm if the customer has tried paraffin sections, for which we can provide the guarantee?

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab94585,ab124025and ab31928. I would also appreciate if you can confirm some further details. There are similar comments to provide for all three antibodies:

1. Fixation 1 hour for frozen sections is a very long time. For Frozen sections, we recommend fixation for 10 minutes only. Otherwize there may be too much protein crosslinking from the PFA, and the epitope may not be accessible to the antibody.

2. As far as I am aware, Recoverin, GABA A Receptor alpha 1 and Neurokinin 3 Receptor NK3 are all membrane or membraneassociated protein.Detection of thesewill not require permeabilization. Indeed, Triton is a strong detergent and can disrupt membrane proteins and eptiopes, which can significantly affect the staining. Therefore, I can suggest to remove the permeabilization step.

3. I can recommend to use a more gentle detergent, such as Tween 20 in the other buffers, try 0.1% - 0.2% Tween.

4. I recommend to include 0.1 - 0.2% tween in the wash buffer, this will help to wash away any excess antibody.

5. We recommend not to mix blocking agents in an experiment. try serum only.

6. Could you confirm, are the current vials of secondary antibodies working well with other primary antibodies? What were the results of the no primary controls? The concentration of secondary antibody may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details, including information regarding ab11259.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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