Product nameAnti-GABA A Receptor alpha 1 antibody [N95/35]
See all GABA A Receptor alpha 1 primary antibodies
DescriptionMouse monoclonal [N95/35] to GABA A Receptor alpha 1
Specificityab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
- WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.
The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab94585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.1 - 1 µg/ml.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Involvement in diseaseDefects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.
Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg
Sheep Anti-Mouse IgG: HRP
Predicted band size: 52 kDa
Block: 1% BSA.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
This product has been referenced in:
- Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . Read more (PubMed: 25987849) »
- Tan S et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . Read more (PubMed: 21712993) »