Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)

Overview

  • Product name
    Anti-GABA A Receptor alpha 1 antibody [N95/35]
    See all GABA A Receptor alpha 1 primary antibodies
  • Description
    Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
  • Host species
    Mouse
  • Specificity
    ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Fusion protein corresponding to Mouse GABA A Receptor alpha 1 aa 355-394.
    Sequence:

    KVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATI


    Database link: P62812

  • Positive control
    • WB: Human cell line mix lysate. IHC-P: Mouse back skin tissue.
  • General notes

    The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

  • Function
    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Involvement in disease
    Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
    Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ECA4 antibody
    • EIEE19 antibody
    • EJM antibody
    • EJM5 antibody
    • Gaba receptor alpha 1 polypeptide antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit alpha 1 antibody
    • GABA(A) receptor subunit alpha-1 antibody
    • GABA(A) receptor, alpha 1 antibody
    • GABRA 1 antibody
    • GABRA1 antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody
    • Gamma aminobutyric acid A receptor alpha 1 antibody
    • Gamma aminobutyric acid receptor subunit alpha 1 antibody
    • Gamma aminobutyric acid type A receptor alpha1 subunit antibody
    • Gamma-aminobutyric acid receptor subunit alpha-1 antibody
    • GBRA1_HUMAN antibody
    see all

Images

  • Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.

    Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.

  • Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP

    Predicted band size: 52 kDa



    Block: 1% BSA.

    Primary antibody incubated for 2 hours at RT.

    Secondary antibody incubated for 1 hour at RT.

References

This product has been referenced in:
  • Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . Read more (PubMed: 25987849) »
  • Tan S  et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . Read more (PubMed: 21712993) »

See all 2 Publications for this product

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