Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585)
Key features and details
- Mouse monoclonal [N95/35] to GABA A Receptor alpha 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-GABA A Receptor alpha 1 antibody [N95/35]
See all GABA A Receptor alpha 1 primary antibodies -
Description
Mouse monoclonal [N95/35] to GABA A Receptor alpha 1 -
Host species
Mouse -
Specificity
ab94585 does not react with GABA A Receptor Alpha 2 or Alpha 3. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment within Mouse GABA A Receptor alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P62812 -
Positive control
- WB: Rat brain normal tissue lysate - membrane extract (ab29473), Human cell line mix lysate. IHC-P: Mouse back skin tissue.
-
General notes
The clone number has been updated from S95-35 to N95/35, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N95/35 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
|
|
IHC-P |
Use a concentration of 0.1 - 1 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
IHC-P
Use a concentration of 0.1 - 1 µg/ml. |
Target
-
Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2554 Human
- Entrez Gene: 14394 Mouse
- Entrez Gene: 29705 Rat
- Omim: 137160 Human
- SwissProt: P08219 Cow
- SwissProt: P14867 Human
- SwissProt: P62812 Mouse
- SwissProt: P62813 Rat
see all -
Alternative names
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
Images
-
Bouin's Fixative and paraffin-embedded mouse back skin tissue stained for GABA A Receptor alpha 1 with ab94585 (1/100 dilution for 1 hour at RT) in immunohistochemical analysis.
Secondary antibody is a FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
-
Anti-GABA A Receptor alpha 1 antibody [N95/35] (ab94585) at 1/1000 dilution + Human cell line mix lysates at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 52 kDaBlock: 1% BSA.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (7)
ab94585 has been referenced in 7 publications.
- Heiney K et al. Neuronal avalanche dynamics and functional connectivity elucidate information propagation in vitro. Front Neural Circuits 16:980631 (2022). PubMed: 36188125
- Zhou Y et al. Soluble β-amyloid impaired the GABA inhibition by mediating KCC2 in early APP/PS1 mice. Biosci Trends 15:330-340 (2021). PubMed: 34526443
- Bian Z et al. Mechanisms Underlying the Action of Ziziphi Spinosae Semen in the Treatment of Insomnia: A Study Involving Network Pharmacology and Experimental Validation. Front Pharmacol 12:752211 (2021). PubMed: 35002696
- Bu X et al. 1% Isoflurane and 1.2 µg/ml of Propofol: A Combination of Anesthetics That Causes the Least Damage to Hypoxic Neurons. Front Aging Neurosci 12:591938 (2020). PubMed: 33304268
- Gong JL et al. The increased expression of GABA receptors within the arcuate nucleus is associated with high intraocular pressure. Mol Vis 24:574-586 (2018). PubMed: 30174387
- Zhao J & Hedera P Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins, are Binding Partners. J Exp Neurosci 9:15-25 (2015). ICC . PubMed: 25987849
- Tan S et al. Gene Expression Changes in GABA(A) Receptors and Cognition Following Chronic Ketamine Administration in Mice. PLoS One 6:e21328 (2011). WB ; Mouse . PubMed: 21712993