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Neuroscience Neurotransmitter Amino Acids GABA
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Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab153980)

  • Datasheet
  • SDS
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Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab153980)

    Key features and details

    • Rabbit polyclonal to GABA A Receptor alpha 2/GABRA2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-GABA A Receptor alpha 2/GABRA2 antibody
      See all GABA A Receptor alpha 2/GABRA2 primary antibodies
    • Description

      Rabbit polyclonal to GABA A Receptor alpha 2/GABRA2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
    • Immunogen

      Recombinant fragment corresponding to Human GABA A Receptor alpha 2/GABRA2 aa 1-330.
      Database link: P47869

    • Positive control

      • NT2D1 cell lysate.
    • General notes

       This product was previously labelled as GABA A Receptor alpha 2

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • GABA
      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • Ligand-Gated Ion Channels
      • GABA Receptors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab153980 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/500 - 1/3000. Predicted molecular weight: 51 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 51 kDa.

    Target

    • Function

      GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
    • Sequence similarities

      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
    • Cellular localization

      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Target information above from: UniProt accession P47869 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282236 Cow
      • Entrez Gene: 2555 Human
      • Entrez Gene: 14395 Mouse
      • Entrez Gene: 289606 Rat
      • Omim: 137140 Human
      • SwissProt: P10063 Cow
      • SwissProt: P47869 Human
      • SwissProt: P26048 Mouse
      • SwissProt: P23576 Rat
      • Unigene: 116250 Human
      • Unigene: 5304 Mouse
      • Unigene: 48180 Rat
      see all
    • Alternative names

      • GABA A receptor subunit alpha 2 antibody
      • GABA antibody
      • GABA(A) receptor subunit alpha 2 antibody
      • GABA(A) receptor subunit alpha-2 antibody
      • GABR A2 antibody
      • GABRA 2 antibody
      • GABRA2 antibody
      • GABRA2 protein antibody
      • Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
      • Gamma aminobutyric acid A receptor alpha 2 antibody
      • Gamma aminobutyric acid receptor alpha 2 subunit antibody
      • Gamma aminobutyric acid receptor subunit alpha 2 antibody
      • Gamma-aminobutyric acid receptor subunit alpha-2 antibody
      • GBRA2_HUMAN antibody
      see all

    Images

    • Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab153980)
      Western blot - Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab153980)
      Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab153980) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

      Predicted band size: 51 kDa



      10% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153980? Please let us know so that we can cite the reference in this datasheet.

    ab153980 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-GABA A Receptor alpha 2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Xenopus laevis Cell lysate - whole cell (Xenopus optic tectum)
    Specification
    Xenopus optic tectum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2015

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