Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab216973)
Key features and details
- Rabbit polyclonal to GABA A Receptor alpha 2/GABRA2
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-GABA A Receptor alpha 2/GABRA2 antibody
See all GABA A Receptor alpha 2/GABRA2 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor alpha 2/GABRA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide within Human GABA A Receptor alpha 2/GABRA2 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P47869 -
Positive control
- WB: Mouse brain cell lysate. Flow Cyt (Intra): RSC96 cells. IHC-P: Rat brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 51 kDa.
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Flow Cyt (Intra) |
1/20 - 1/100.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 51 kDa. |
Flow Cyt (Intra)
1/20 - 1/100. |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2555 Human
- Entrez Gene: 14395 Mouse
- Entrez Gene: 289606 Rat
- Omim: 137140 Human
- SwissProt: P47869 Human
- SwissProt: P26048 Mouse
- SwissProt: P23576 Rat
- Unigene: 116250 Human
see all -
Alternative names
- GABA A receptor subunit alpha 2 antibody
- GABA antibody
- GABA(A) receptor subunit alpha 2 antibody
see all
Images
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Anti-GABA A Receptor alpha 2/GABRA2 antibody (ab216973) at 1/300 dilution + Mouse brain cell lysate
Secondary
conjugated secondary at 1/5000 dilution
Predicted band size: 51 kDa -
Intracellular flow cytometric analysis of RSC96 cells labeling GABA A Receptor alpha 2/GABRA2 with ab216973 at 1/100 dilution followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Rat brain tissue labeling GABA A Receptor alpha 2/GABRA2 with ab216973 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216973 has not yet been referenced specifically in any publications.