Product nameAnti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19]
See all GABA A Receptor alpha 2/GABRA2 primary antibodies
DescriptionMouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Rat GABA A Receptor alpha 2/GABRA2 aa 350-450.
- ICC/IF: SKNSH cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab193311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52,104 kDa (predicted molecular weight: 51 kDa).|
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- GABA A receptor subunit alpha 2 antibody
- GABA antibody
- GABA(A) receptor subunit alpha 2 antibody
All lanes : Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : Pancreas (Rat) Tissue Lysate
Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
Lane 6 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Additional bands at: 104 kDa (possible dimer)
Exposure time: 20 minutes
ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This product has been referenced in:
- Zhang W et al. Effect of Yi-nao-jie-yu decoction on ?-aminobutyric acid type A receptor in the hippocampus and serum inflammatory factors in a rat model of poststroke anxiety. Neuropsychiatr Dis Treat 12:2827-2837 (2016). WB ; Rat . Read more (PubMed: 27843317) »