• Product name

    Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19]
    See all GABA A Receptor alpha 2/GABRA2 primary antibodies
  • Description

    Mouse monoclonal [N399/19] to GABA A Receptor alpha 2/GABRA2
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat GABA A Receptor alpha 2/GABRA2 aa 350-450.

  • Positive control

    • ICC/IF: SKNSH cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

     This product was previously labelled as GABA A Receptor alpha 2




Our Abpromise guarantee covers the use of ab193311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 52,104 kDa (predicted molecular weight: 51 kDa).


  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GABA A receptor subunit alpha 2 antibody
    • GABA antibody
    • GABA(A) receptor subunit alpha 2 antibody
    • GABA(A) receptor subunit alpha-2 antibody
    • GABR A2 antibody
    • GABRA 2 antibody
    • GABRA2 antibody
    • GABRA2 protein antibody
    • Gamma aminobutyric acid (GABA) A receptor alpha 2 antibody
    • Gamma aminobutyric acid A receptor alpha 2 antibody
    • Gamma aminobutyric acid receptor alpha 2 subunit antibody
    • Gamma aminobutyric acid receptor subunit alpha 2 antibody
    • Gamma-aminobutyric acid receptor subunit alpha-2 antibody
    • GBRA2_HUMAN antibody
    see all


  • All lanes : Anti-GABA A Receptor alpha 2/GABRA2 antibody [N399/19] (ab193311) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Pancreas (Rat) Tissue Lysate
    Lane 5 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 6 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Additional bands at: 104 kDa (possible dimer)

    Exposure time: 20 minutes
  • ICC/IF image of ab193311 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab193311 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.


This product has been referenced in:

  • Zhang W  et al. Effect of Yi-nao-jie-yu decoction on ?-aminobutyric acid type A receptor in the hippocampus and serum inflammatory factors in a rat model of poststroke anxiety. Neuropsychiatr Dis Treat 12:2827-2837 (2016). WB ; Rat . Read more (PubMed: 27843317) »
See 1 Publication for this product

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