Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446)

Submit a review Q&A (9)References (3)
Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446)

    Key features and details

    • Rabbit polyclonal to GABA A Receptor alpha 3/GABRA3
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    Overview

    • Product name

      Anti-GABA A Receptor alpha 3/GABRA3 antibody
      See all GABA A Receptor alpha 3/GABRA3 primary antibodies

    • Description

      Rabbit polyclonal to GABA A Receptor alpha 3/GABRA3

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse, Rat

    • Immunogen

      Fusion protein corresponding to Rat GABA A Receptor alpha 3/GABRA3 (N terminal).

    • General notes

       This product was previously labelled as GABA A Receptor alpha 3

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab72446 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

    Target

    • Function

      GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

    • Sequence similarities

      Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA3 sub-subfamily.

    • Cellular localization

      Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • GABA A Receptor alpha 3 antibody
        • GABA(A) receptor subunit alpha 3 antibody
        • GABA(A) receptor subunit alpha-3 antibody
        • GABR A3 antibody
        • GABRA 3 antibody
        • Gabra3 antibody
        • Gamma aminobutyric acid (GABA) A receptor alpha 3 antibody
        • Gamma aminobutyric acid A receptor alpha 3 antibody
        • Gamma aminobutyric acid receptor subunit alpha 3 antibody
        • Gamma-aminobutyric acid receptor subunit alpha-3 antibody
        • GBRA3_HUMAN antibody
        • MGC33793 antibody
        see all

      Images

      • Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446)
        Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446)
        All lanes : Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446) at 1/1000 dilution

        Lane 1 : wild type rat brain lysates
        Lane 2 : GABA A Receptor alpha 3/GABRA3 knock out rat brain lysates

        Predicted band size: 55 kDa
        Observed band size: 55 kDa

      References (3)

      Publishing research using ab72446? Please let us know so that we can cite the reference in this datasheet.

      ab72446 has been referenced in 3 publications.

      • Long M  et al. miR-92b-3p acts as a tumor suppressor by targeting Gabra3 in pancreatic cancer. Mol Cancer 16:167 (2017). PubMed: 29078789
      • Kaneko Y  et al. Oxytocin modulates GABAAR subunits to confer neuroprotection in stroke in vitro. Sci Rep 6:35659 (2016). WB ; Rat . PubMed: 27767042
      • Bates RC  et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). PubMed: 24836856

      Customer reviews and Q&As

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      1-9 of 9 Abreviews or Q&A

      Question

      Hi , I tried to use mouse sample this time and the antibody still cannot work. The attachment are two pictures taken from same membrane. The right three lanes in pic1 were bond with alpha3. The signal was very light. Using the same membrane, pic 2 shows staining with GABA receptor alpha1 antibody that we got it from Millipore. So, it is not because species, the antibody may have some problem. We purchased in May last year.

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      Answer

      Thank you for your response. I am sorry to hear of this continued difficulty.

      The images that you mentioned were not included in this email. Would you be able to re-send those to me?



      As always, this product is covered by our Abpromise guarantee.

      If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, if used according to specifications listed on our datasheet.



      Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More

      Question

      Thank you for the email. Sorry I missed your call.


      1) What kind of samples were used (species and cell/tissue type)?
      We used this previous antibody on ferret brain tissue and mouse brain tissue for WB
      2) What dilution of the primary antibody was used?
      I used the dilution of 1:1000
      3) What blocking solution was used (BSA, milk, etc)?
      I used 5% milk for blocking.

      Thank you. I just want to you know I also use some other antibodies, for example anti-GABAA alpha 1, anti-GABAA gamma2 2, anti-GAD etc, the other antibodies work well on our ferret and mouse brain samples.

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      Answer

      Thank you for your reply and for sending the information.

      The protocol information will be very helpful to us. Please keep me updated about the results using the replacement antibody, and let me know if there is anything else that we can do for you.

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      Question

      Ab72446 did not work in Western blot.

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      Answer

      Thanks for your call today.

      As you requested, I'm sending a vial of ab23703 (Anti-Cannabinoid Receptor I antibody) free of charge on the order ***. This order will ship out tonight and get to you tomorrow.

      Would you mindbriefly answering the questions below about the protocol that was used with ab72446? We monitor all product complaints and would like to investigate the trouble with this product.

      1) What kind of samples were used (species and cell/tissue type)?
      2) What dilution of the primary antibody was used?
      3) What blocking solution was used (BSA, milk, etc)?

      I look forward to hearing from, either by email or phone. Please let me know if you have any questions or if there is anything else that we can do for you.

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      Question

      Thank you for your response. I am sorry to hear of this continued difficulty.

      Read More
      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab72446.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Thank you for your response. I am sorry to hear of this continued difficulty.

      The images that you mentioned were not included in this email. Would you be able to re-send those to me?



      As always, this product is covered by our Abpromise guarantee.

      If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, if used according to specifications listed on our datasheet.



      Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More
      Answer

      Thanks for re-sending the images.

      If you feel the vial of ab72446 is not performing as expected, I would be happy to offer a replacement of any primary antibody, credit or refund. Typically our guarantee extends to 6 months from receipt but in this case I can make an exception.

      In your reply, please include your PO number or Abcam order reference number so I can process your request.

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      Question

      Willab72446 cross react with ferret protein?

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      Answer

      Thank you for your enquiry.

      I have been unable to locate the ferret protein sequence for GABA A Receptor alpha 3. If you have it or know where I can find it please let me know. I can then forward this sequence information to the lab for an alignment to assess likelihood of cross reactivity with ferret protein.

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      Question

      What was the IHC-Fr protocol that was used.  

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      Answer

      Thank you for your reply. The anti-GABA A Receptor alpha 3 antibody, ab72446, is covered under our Abpromise and so is guaranteed to work in IHC-Fr on mouse tissue. If the antibody fails to perform as stated and if the scientific support team cannot help resolve this issue, then we would be happy to provide you with an alternative antibody or a refund. I have emailed the lab to request more information as the protocol used in testing ab72446 in IHC-Fr, and they have told me that the only information they have is what I have already sent you. . The lab did inform me that this antibody had been published in papers from Dr. Werner Sieghart lab and so searching his publications may yield finer protocol details. The protocol guides that I sent along yesterday should be able to provide you with more information on general IHC techniques Please let me know if there is anything else I can do to help. .

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      Question

      What was the IHC-Fr protocol that was used.  

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      Answer

      Thank you for contacting Abcam. The lab has gotten back to my request for more information on the IHC protocol that was used to test ab72446. Below is the protocol that they have sent me. I have also attached Abcams general IHC and IHC-Fr protocol guides. If there is anything else I can help you with, please let me know. Basic protocol used for IHC with ab72446 Fixation: 4% PFA + 15% picric acid Sections: vibratome, 50 um Method: free-floating blocking: 20% normal horse serum Primary (1:100) and Secondary (manufacturer's recommended) Ab dilution: in 2% normal horse serum

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      Question

      What was the IHC-Fr protocol that was used.  

      Read More
      Answer

      Thank you for contacting Abcam. The only information I have been able to find about the IHC-Fr protocol use so far is that it was tested at 1/100 against on Rat amygdala (4% PFA + 15% picric acid fixed frozen sections. I am going to email the lab for more information and I will send that information onto you as soon as I get it. If there is anything else I can help you with please let me know.

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