Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab72446)

Thank you for your response. I am sorry to hear of this continued difficulty.

The images that you mentioned were not included in this email. Would you be able to re-send those to me?



As always, this product is covered by our Abpromise guarantee.

If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, if used according to specifications listed on our datasheet.



Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for your reply and for sending the information.

The protocol information will be very helpful to us. Please keep me updated about the results using the replacement antibody, and let me know if there is anything else that we can do for you.

Thanks for your call today.

As you requested, I'm sending a vial of ab23703 (Anti-Cannabinoid Receptor I antibody) free of charge on the order ***. This order will ship out tonight and get to you tomorrow.

Would you mindbriefly answering the questions below about the protocol that was used with ab72446? We monitor all product complaints and would like to investigate the trouble with this product.

1) What kind of samples were used (species and cell/tissue type)?
2) What dilution of the primary antibody was used?
3) What blocking solution was used (BSA, milk, etc)?

I look forward to hearing from, either by email or phone. Please let me know if you have any questions or if there is anything else that we can do for you.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab72446.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thanks for re-sending the images.

If you feel the vial of ab72446 is not performing as expected, I would be happy to offer a replacement of any primary antibody, credit or refund. Typically our guarantee extends to 6 months from receipt but in this case I can make an exception.

In your reply, please include your PO number or Abcam order reference number so I can process your request.

Thank you for your enquiry.

I have been unable to locate the ferret protein sequence for GABA A Receptor alpha 3. If you have it or know where I can find it please let me know. I can then forward this sequence information to the lab for an alignment to assess likelihood of cross reactivity with ferret protein.

Thank you for your reply. The anti-GABA A Receptor alpha 3 antibody, ab72446, is covered under our Abpromise and so is guaranteed to work in IHC-Fr on mouse tissue. If the antibody fails to perform as stated and if the scientific support team cannot help resolve this issue, then we would be happy to provide you with an alternative antibody or a refund. I have emailed the lab to request more information as the protocol used in testing ab72446 in IHC-Fr, and they have told me that the only information they have is what I have already sent you. . The lab did inform me that this antibody had been published in papers from Dr. Werner Sieghart lab and so searching his publications may yield finer protocol details. The protocol guides that I sent along yesterday should be able to provide you with more information on general IHC techniques Please let me know if there is anything else I can do to help. .

Thank you for contacting Abcam. The lab has gotten back to my request for more information on the IHC protocol that was used to test ab72446. Below is the protocol that they have sent me. I have also attached Abcams general IHC and IHC-Fr protocol guides. If there is anything else I can help you with, please let me know. Basic protocol used for IHC with ab72446 Fixation: 4% PFA + 15% picric acid Sections: vibratome, 50 um Method: free-floating blocking: 20% normal horse serum Primary (1:100) and Secondary (manufacturer's recommended) Ab dilution: in 2% normal horse serum

Thank you for contacting Abcam. The only information I have been able to find about the IHC-Fr protocol use so far is that it was tested at 1/100 against on Rat amygdala (4% PFA + 15% picric acid fixed frozen sections. I am going to email the lab for more information and I will send that information onto you as soon as I get it. If there is anything else I can help you with please let me know.