Key features and details
- Mouse monoclonal [S415-24] to GABA A Receptor alpha 5/GABRA5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24]
DescriptionMouse monoclonal [S415-24] to GABA A Receptor alpha 5/GABRA5
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse brain tissue lysate. ICC/IF: SK-N-BE cells.
This product was previously labelled as GABA A Receptor alpha 5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab242001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 52 kDa.|
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- GAA 5 antibody
- GAA5 antibody
- GABA(A) receptor subunit alpha-5 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for GABA A Receptor alpha 5/GABRA5 (green, C) using ab242001 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 min at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.
Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (ab242001) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg
Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 52 kDa
Primary incubation for 16 hours at 4°C.
Secondary incubation for 1 hr at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab242001 has not yet been referenced specifically in any publications.