Recombinant Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069)
![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-485331-ab300069WB1.jpg "Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25322-129] to GABA A Receptor alpha 6
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GABA A Receptor alpha 6 antibody [EPR25322-129]
See all GABA A Receptor alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR25322-129] to GABA A Receptor alpha 6 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human hypothalamus, Mouse brain, Rat brain, Rat cerebellum lysates. IHC-P: Mouse cerebellum, Rat cerebellum and Human cerebellum tissues. IHC-Fr: Mouse cerebellum and Rat cerebellum tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25322-129 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
|
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| IHC-Fr |
1/500.
|
| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa). |
|
IHC-Fr
1/500. |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA6 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2559 Human
- Entrez Gene: 14399 Mouse
- Entrez Gene: 29708 Rat
- Omim: 137143 Human
- SwissProt: Q16445 Human
- SwissProt: P16305 Mouse
- SwissProt: P30191 Rat
- Unigene: 90791 Human
see all -
Alternative names
- GABA A antibody
- GABA A Receptor alpha 6 polypeptide antibody
- GABA A receptor alpha 6 antibody
see all
Images
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Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)All lanes : Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution
Lane 1 : Human hypothalamus tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds5% NFDM/TBST was used as blocking and diluting buffer.
Low expression:Heart (PMID:29467616).
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![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-485333-ab300069WB2.jpg)
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsThis data was developed using ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
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![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-485335-ab300069WB3.jpg)
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)All lanes : Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds5% NFDM/TBST was used as blocking and diluting buffer.
Low expression:Heart (PMID:29467616)
This blot was developed using a high sensitivity ECL substrate. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-5-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-6-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-rat-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-7-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-human-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-8-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-frozen-mouse-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with ab300069 at 1/500 (1.244 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/mL dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 µ/mL dilution.
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![Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-9-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-frozen-rat-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with ab300069 at 1/500 (1.244 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µ/mL) dilution.
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![Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)](https://www.abcam.com/ps/products/300/ab300069/Images/AB300069-485290-Benefits-of-Recombinant-antibodies.jpg)
Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (AB300069)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300069 has not yet been referenced specifically in any publications.