Recombinant Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (ab300070)
![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-485332-ab300069WB1.jpg "Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25322-129] to GABA A Receptor alpha 6 - BSA and Azide free
- Suitable for: WB, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free)
See all GABA A Receptor alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR25322-129] to GABA A Receptor alpha 6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human hypothalamus, Mouse brain, Rat brain, Rat cerebellum lysates. IHC-P: Mouse cerebellum, Rat cerebellum and Human cerebellum tissues. IHC-Fr: Mouse cerebellum and Rat cerebellum tissues.
-
General notes
ab300070 is a carrier free version of ab300069.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25322-129 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa).
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 51 kDa). |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA6 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2559 Human
- Entrez Gene: 14399 Mouse
- Entrez Gene: 29708 Rat
- Omim: 137143 Human
- SwissProt: Q16445 Human
- SwissProt: P16305 Mouse
- SwissProt: P30191 Rat
- Unigene: 90791 Human
see all -
Alternative names
- GABA A antibody
- GABA A Receptor alpha 6 polypeptide antibody
- GABA A receptor alpha 6 antibody
see all
Images
-
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)All lanes : Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution
Lane 1 : Human hypothalamus tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
Low expression:Heart (PMID:29467616).
-
![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-485334-ab300069WB2.jpg)
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsThis data was developed using ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
-
![Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-485336-ab300069WB3.jpg)
Western blot - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)All lanes : Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (ab300069) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Predicted band size: 51 kDa
Exposure time: 180 secondsThis data was developed using ab300069, the same antibody clone in a different buffer formulation.
5% NFDM/TBST was used as blocking and diluting buffer.
Low expression:Heart (PMID:29467616)
This blot was developed using a high sensitivity ECL substrate. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-5-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)This data was developed using ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-6-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-rat-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)This data was developed using ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on rat cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-7-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-human-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)This data was developed using ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling GABA A Receptor alpha 6 with ab300069 at 1/500 dilution (1.244 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human cerebellum. The section was incubated with ab300069 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-8-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-frozen-mouse-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)This data was developed using ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with ab300069 at 1/500 (1.244 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 µg/mL dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 µ/mL dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-9-anti-gaba-a-receptor-alpha-6-antibody-epr25322129-immunohistochemistry-frozen-rat-cerebellum-fresh.jpg)
Immunohistochemistry (Frozen sections) - Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)This data was developed using ab300069, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling GABA A Receptor alpha 6 with ab300069 at 1/500 (1.244 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µ/mL) dilution.
-
![Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)](https://www.abcam.com/ps/products/300/ab300070/Images/AB300070-485280-Benefits-of-Recombinant-antibodies.jpg)
Anti-GABA A Receptor alpha 6 antibody [EPR25322-129] (BSA and Azide free) (AB300070)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab300070 has not yet been referenced specifically in any publications.
