The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
This image shows paraffin-embedded human breast carcinoma tissue stained with ab51123 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
Western blot - Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)
All lanes : Anti-GABA A Receptor beta 1 (phospho S434) antibody (ab51123)
Lane 1 : Extracts from cos7 cells with immunising peptide Lane 2 : Extracts from cos7 cells with no peptide
ICC/IF image of ab51123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.