Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659)
Key features and details
- Rabbit polyclonal to GABA A Receptor beta 3/GABRB3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GABA A Receptor beta 3/GABRB3 antibody
See all GABA A Receptor beta 3/GABRB3 primary antibodies -
Description
Rabbit polyclonal to GABA A Receptor beta 3/GABRB3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit -
Immunogen
Synthetic peptide corresponding to Rat GABA A Receptor beta 3/GABRB3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab125892) -
Positive control
- WB: Rat and mouse brain and hippocampus tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. -
Involvement in disease
Epilepsy, childhood absence 5 -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. -
Cellular localization
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2562 Human
- Entrez Gene: 14402 Mouse
- Entrez Gene: 24922 Rat
- Omim: 137192 Human
- SwissProt: P28472 Human
- SwissProt: P63080 Mouse
- SwissProt: P63079 Rat
- Unigene: 302352 Human
see all -
Alternative names
- ECA5 antibody
- GABA alpha receptor beta-2 subunit antibody
- GABA(A) receptor subunit beta-3 antibody
see all
Images
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All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsGABA A Receptor beta 3/GABRB3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Rat Hippocampus Tissue Lysate
Lane 5 : Mouse Hippocampus Tissue Lysate
Lane 6 : Human hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab104659 has been referenced in 1 publication.
- Oda R et al. Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy. iScience 25:103936 (2022). PubMed: 35252821