Overview

  • Product name

    Anti-GABA A Receptor beta 3/GABRB3 antibody
    See all GABA A Receptor beta 3/GABRB3 primary antibodies
  • Description

    Rabbit polyclonal to GABA A Receptor beta 3/GABRB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide corresponding to Rat GABA A Receptor beta 3/GABRB3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab125892)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat hippocampus; Mouse Hippocampus.
  • General notes

     This product was previously labelled as GABA A Receptor beta 3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
  • Involvement in disease

    Epilepsy, childhood absence 5
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ECA5 antibody
    • GABA alpha receptor beta-2 subunit antibody
    • GABA(A) receptor subunit beta-3 antibody
    • GABAA receptor beta 3 subunit antibody
    • GABAA receptor subunit beta 3 antibody
    • GABR B3 antibody
    • Gabrb3 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 3 antibody
    • Gamma aminobutyric acid receptor subunit beta 3 antibody
    • Gamma-aminobutyric acid receptor subunit beta-3 antibody
    • GBRB3_HUMAN antibody
    • MGC9051 antibody
    see all

Images

  • IHC image of GABA A Receptor beta 3/GABRB3 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Rat Hippocampus Tissue Lysate
    Lane 4 : Mouse Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    GABA A Receptor beta 3/GABRB3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

ab104659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for these particular antibodies as it is proprietary information. Unfortunately, we do not have the corresponding peptides for sale, either.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.


If your customer is interested in antibodies with a given immunogen sequence I would suggest ab123288Anti-GABA A Receptor beta 2 antibody

andab104659 Anti-GABA A Receptor beta 3 antibody( for this product, we have the peptide available).


Should you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up