Recombinant Anti-GABA A Receptor beta 2/GABRB2 + GABA A Receptor gamma 2/GABRG2 + GABA A Receptor alpha 1 antibody [1F4] (ab281915)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [1F4] to GABA A Receptor beta 2/GABRB2 + GABA A Receptor gamma 2/GABRG2 + GABA A Receptor alpha 1
- Suitable for: ICC
- Reacts with: Human
Overview
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Product name
Anti-GABA A Receptor beta 2/GABRB2 + GABA A Receptor gamma 2/GABRG2 + GABA A Receptor alpha 1 antibody [1F4] -
Description
Rabbit monoclonal [1F4] to GABA A Receptor beta 2/GABRB2 + GABA A Receptor gamma 2/GABRG2 + GABA A Receptor alpha 1 -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. Raised against alpha1 beta2 gamma2 GABA-A receptor.
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Positive control
- ICC: U251 cells.
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General notes
ab281915 is the rabbit chimera vesion of ab281914.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1F4 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 10 µg/ml.
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Notes |
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ICC
Use a concentration of 10 µg/ml. |
Target
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Cellular localization
GABA A Receptor beta 2/GABRB2: Cell junction > synapse > postsynaptic cell membrane. Cell membrane. GABA A Receptor gamma 2/GABRG2: Cell junction > synapse > postsynaptic cell membrane. Cell membrane. GABA A Receptor alpha 1: Cell junction > synapse > postsynaptic cell membrane. Cell membrane. -
Database links
- Entrez Gene: 2554 Human
- Entrez Gene: 2561 Human
- Entrez Gene: 2566 Human
- Omim: 137160 Human
- Omim: 137164 Human
- Omim: 600232 Human
- SwissProt: P14867 Human
- SwissProt: P18507 Human
see all
Images
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U251 cells stained for GABA A Receptor gamma 2/GABRG2 + GABA A Receptor alpha 1 + GABA Receptor Beta 2 (green) using ab281915 at 10 μg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml in ICC. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab281915, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab281915 has been referenced in 1 publication.
- Zhu S et al. Structure of a human synaptic GABAA receptor. Nature 559:67-72 (2018). PubMed: 29950725