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Synthetic peptide within Human GABA A Receptor gamma 3 aa 8-37 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q99928
Our Abpromise guarantee covers the use of ab174242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 54 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Flow cytometric analysis of K562 cells (bottom histogram) labeling GABA A Receptor gamma 3 using ab174242 at 1/10 dilution compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis in formalin fixed and paraffin embedded brain tissue labeling GABA A Receptor gamma 3 using ab174242 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab174242 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"