Overview

  • Product name
    Anti-GABA A Receptor gamma 3 antibody - N-terminal
  • Description
    Rabbit polyclonal to GABA A Receptor gamma 3 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human GABA A Receptor gamma 3 aa 8-37 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q99928

  • Positive control
    • Mouse brain lysate and tissue, K562 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.9% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174242 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    GABA A Receptor gamma 3, is the ligand-gated ion channel that GABA A (gamma aminobutyric acid) exerts its inhibitory neurotransmitter funtion through.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit gamma 3 antibody
    • GABR G3 antibody
    • GABRG 3 antibody
    • GABRG3 antibody
    • Gamma aminobutyric acid (GABA) A receptor gamma 3 antibody
    • Gamma aminobutyric acid A receptor gamma 3 antibody
    • Gamma aminobutyric acid receptor subunit gamma 3 antibody
    see all

Images

  • Flow cytometric analysis of K562 cells (bottom histogram) labeling GABA A Receptor gamma 3 using ab174242 at 1/10 dilution compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-GABA A Receptor gamma 3 antibody - N-terminal (ab174242) at 1/100 dilution + Mouse brain tissue lysate at 35 µg

    Predicted band size: 54 kDa

  • Immunohistochemical analysis in formalin fixed and paraffin embedded brain tissue labeling GABA A Receptor gamma 3 using ab174242 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab174242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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