Anti-GABA antibody (ab17413)
Key features and details
- Guinea pig polyclonal to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISA
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GABA antibody
See all GABA primary antibodies -
Description
Guinea pig polyclonal to GABA -
Host species
Guinea pig -
Specificity
Recognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining. -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-FoFr, IHC-Fr, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to GABA conjugated to bovine serum albumin (Glutaraldehyde).
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General notes
Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
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If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
Without colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/1000.
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IHC-FoFr | (2) |
1/1000.
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IHC-Fr | (2) |
1/1500.
|
ELISA |
Use at an assay dependent concentration.
|
Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
IHC-FoFr
1/1000. |
IHC-Fr
1/1500. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
Cellular localization
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
Alternative names
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
Images
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Immunohistochemical analysis of rat brain tissue, labelling GABA with ab17413 diluted 1/500. Heat-induced antigen retrieval. DAB staining. Diffuse cytoplasmic staining and axonal staining observed in ventral midbrain neurons. (Note: This antibody produces diffuse cytoplasmic staining of moderate intensity in ventral midbrain, cortical and purkinje neurons in rat brain tissue).
Protocols
Datasheets and documents
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Datasheet download
References (26)
ab17413 has been referenced in 26 publications.
- Yoo YM et al. Amylin Protein Expression in the Rat Brain and Neuro-2a Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35457166
- Stumpner A et al. Auditory DUM neurons in a bush-cricket: inhibited inhibitors. J Comp Physiol A Neuroethol Sens Neural Behav Phys 206:793-807 (2020). PubMed: 32656577
- Li C et al. Downregulation of microRNA-29c reduces pain after child delivery by activating the oxytocin-GABA pathway. Mol Med Rep 22:1921-1931 (2020). PubMed: 32705167
- Park SY et al. Distribution of and steroid hormone effects on calbindin-D9k in the immature rat brain. Brain Res Bull 152:225-235 (2019). PubMed: 31357009
- Leroy F et al. A circuit from hippocampal CA2 to lateral septum disinhibits social aggression. Nature 564:213-218 (2018). PubMed: 30518859