Overview

  • Product name

  • Description

    Chicken polyclonal to GABA
  • Host species

    Chicken
  • Specificity

    Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against: L-alanine, D/L-aspartate, agmatine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-histidine, L-isoleucine, L-lysine, L-leucine, L-methionine, L-asparagine, L-ornithine, L-proline, L-serine, taurine, L-threonine, L-valine, L-tryptophan, L-tyrosine. No measurable reactivity against formaldehyde fixed proteins
  • Tested applications

    Suitable for: ICCmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    4-aminobutyrate cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Thimerosal (merthiolate)
    Constituent: 0.268% PBS
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    IgYs were separated from other yolk proteins by serial PEG6000 extractions using a modification of the method of Polsonet al Immunological Investigations.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
  • Application notes
    Endogenous content mapping by LM and EM ICC: 1/100. True dilution at user dilution: 1/32000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
    • Cellular localization

      Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
    • Alternative names

      • 4 aminobutanoic acid antibody
      • GABA antibody
      • Gamma amino butyric acid antibody
      • Gamma aminobutyric acid antibody

    References

    This product has been referenced in:

    • Ausborn J  et al. Organization of the core respiratory network: Insights from optogenetic and modeling studies. PLoS Comput Biol 14:e1006148 (2018). Read more (PubMed: 29698394) »
    • Estakhr J  et al. Differential Control of Dopaminergic Excitability and Locomotion by Cholinergic Inputs in Mouse Substantia Nigra. Curr Biol 27:1900-1914.e4 (2017). Read more (PubMed: 28648825) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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