Product nameAnti-GABA antibody
See all GABA primary antibodies
DescriptionRabbit polyclonal to GABA
SpecificityUsing a conjugate GABA-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
(a): GABA-G-BSA concentration/unconjugated or conjugated aminoacid concentration at half displacement. G = Glutaraldehyde, BSA = bovine serum albumin.
Compound Cross-reactivity ratio (a) GABA-G-BSA 1 beta-Alanine-G-BSA 1/10000 Glycine-G-BSA 1/20000 D-Aspartate-G-BSA 1/100000 D-Glutamate-G-PPL 1/>10000
Tested applicationsSuitable for: ELISA, IHC-Frmore details
Chemical/ Small Molecule by a Glutaraldehyde linker.
The antiserum was tested using the free floating PAP technique on rat hippocampus and cerebellum. The anti-conjugated GABA antibodies gave a good staining between 1/2000 - 1/5000 dilution in these areas.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Primary antibody notesThe antiserum was tested using the free floating PAP technique on rat hippocampus and cerebellum. The anti-conjugated GABA antibodies gave a good staining between 1/2000 - 1/5000 dilution in these areas.
Our Abpromise guarantee covers the use of ab6443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceGamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
Cellular localizationCell Membrane, Cytoplasmic, Plasma membrane and Secreted
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
ab6443 has not yet been referenced specifically in any publications.