Product nameAnti-GABA antibody
See all GABA primary antibodies
DescriptionRabbit polyclonal to GABA
Specificityab8891 targets conjugated GABA (Gamma-Aminobutyric acid), it does not recognize free GABA.
Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-FoFrmore details
Chemical/ Small Molecule corresponding to GABA. Synthetic GABA conjugated to bovine serum albumin.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: ddH20, 50% Glycerol
Our Abpromise guarantee covers the use of ab8891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Fixation of tissue for use with these antibodies should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 17394161|
RelevanceGamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
Cellular localizationCell Membrane, Cytoplasmic, Plasma membrane and Secreted
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
Immunohistochemical analysis of rat brain tissue, staining GABA with ab8891.
Slices were fixed in a 2% glutaraldehyde, 2% formaldehyde solution before being blocked for 2 h at room temperature in PBS with 0.3% Tween-20, 0.2% BSA and 5% normal goat serum. Samples were incubated with primary antibody (1/200) before detection with a fluorescence-conjugated goat anti-rabbit secondary antibody.
Immunofluorescent analysis of Human embryonic stem cells, staining GABA with ab8891.
Cells were fixed with paraformaldehyde before blocking with 10% goat serum, 1% BSA, 0.1% Triton X-100 for 1 hour. Samples were stained with primary antibody (1/500) overnight at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
This product has been referenced in:
- Zieger E et al. Roles of Retinoic Acid Signaling in Shaping the Neuronal Architecture of the Developing Amphioxus Nervous System. Mol Neurobiol N/A:N/A (2017). Read more (PubMed: 28875454) »
- Ogundele OM et al. Systemic Sympathoexcitation Was Associated with Paraventricular Hypothalamic Phosphorylation of Synaptic CaMKIIa and MAPK/ErK. Front Neurosci 11:447 (2017). Read more (PubMed: 28824368) »