Key features and details
- Rabbit polyclonal to GABA
- Suitable for: IHC-FoFr, IHC-Fr, Immunomicroscopy
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-GABA antibody
See all GABA primary antibodies
DescriptionRabbit polyclonal to GABA
SpecificityThe antibody is calibrated against a spectrum of antigens to assure hapten selectivity. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine,D/L-aspartate,1-amino-4-guanidobutane (AGB), D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, Immunomicroscopymore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule conjugated to BSA by a Glutaraldehyde linker
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recommended user dilution: 1/100
True Dilution at User Dilution: 1/32000
High-performance thin section immunostaining using silver-intensified immunogold or fluorescence detection.
Enhanced detection is possible using streptavidin detection.
EM applications with 10-40 nm gold GAR IgGs or gold streptavidin.
OPTIMAL FIXATION: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten).
MINIMAL GLUTARALDEHYDE: 0.00% using minimum glutaraldehyde Enhanced HPI (EHPI) protocol with 4% formaldehyde (see "Protocols" tab above)
The hapten is osmium tolerant (deosmication required), therefore the antisera can be used on conventional post-embedding electron microscope immunostaining.
All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.
Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.
RelevanceGamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
Cellular localizationCell Membrane, Cytoplasmic, Plasma membrane and Secreted
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ab9446 has been referenced in 3 publications.
- Shivashankar G et al. Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions. Mol Vis 26:277-290 (2020). PubMed: 32300272
- Chang LY et al. Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus. Front Neurosci 14:161 (2020). PubMed: 32256305
- Sun D et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC ; Rat . PubMed: 17729258