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After a few optimization process, I have attached pictures of WB with ab75239 (and ab55051 if it helps you) for abreview. In both cases, samples are the same (rat brain homogenates). We purchased ab55051 first and we were upset with so many bands. So, you kindly sent me ab75239 for abreview. With both Abs, there are 4-5 bands, including the ones around 95kDa & 130kDa. I think these bands are GABAb-R1B/C/D & -R1A/E since GABAb-Rs are supposed to be R1D (91kDa), R1B (95kDa), R1C (98kDa), and & R1A (108kDa), R1E (12kDa). If I should change something or providew you additional information, please let me know. Thank you for your kindness again. PS: We are out of BCA Kit now and ordering it. So, we don't know the [protein] at all. Instead, I calculated wet weight of brain tissue before homogenization in RIPA buffer. Once BCA kit comes, I can give you the estimated [protein].
Asked on Sep 07 2011
Thanks so much for sending the results and for keeping me updated. I'm sorry to see that the antibody is still picking up so many bands, however you may be correct that some of the bands are isoforms of the target protein. In order for the Abreview to display on the datasheet, the information needs to be submitted at the "Submit an Abreview" link on our datasheet. This link will take you to a form with questions about your protocol, and you can also add the image to the review. If you have any questions about the review form, please let me know and I'd be happy to help. Let me know if you have any questions or if there is anything else that we can do for you.
Answered on Sep 07 2011