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Hello, We are interested in ordering antibodies for the flow cytometry and I would like to make sure that antibody can get an access to the receptor subunits. Another requirement is that they need to react with human cells. The antibodies we are interested are the following Anti GABA A receptor alpha 1 subunit antibody https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html Anti GABA A receptor beta 2 subunit antibody https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html Anti GABA B receptor 1 antibody https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html Can you please verify that they will work with flow cytometry before I place an order? Could you please also provide us a quote for the antibodies? Thank in advance. We appreciate your help. Best wishes,
Asked on Apr 24 2012
Thank you for contacting us.
I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.
Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) and ab86320 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab86320.html) will both recognize the extracellular region of this protein in human tissue. Each will work as well as the other with the only difference being that ab86320 was created fromHuman GABA A Receptor alpha 1 andhas 100% homology, while ab33299 is created fromRat GABA A Receptor alpha 1with 93% homology.
Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html)is raised against the extracellular region of this protein and will react with human tissues.
Anti GABA B receptor 1 antibody: ab90883 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html) recognizes a cytoplasmic region of this protein and will require permeabilzation of the cells to be effective. Alternately ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html) recognizes the extracellular region of this protein. This will also work with human tissues.
None of our antibodies against these targets have been tested in flow cytometry. As such I may offer you enrollment in our 100% Abreview Testing Discount. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
I hope this information is helpful to you. Please let me know if you have any questions or would like to take advantage of our testing discount with these products.
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Answered on Apr 24 2012