Anti-GABA B Receptor 1 antibody (ab55051)
- Datasheet
- References (28)
- Protocols
Overview
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Product nameAnti-GABA B Receptor 1 antibody
See all GABA B Receptor 1 primary antibodies -
DescriptionMouse monoclonal to GABA B Receptor 1
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-FoFr, IHC-Fr, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment: AVYIGALFPM SGGWPGGQAC QPAVEMALED VNSRRDILPD YELKLIHHDS KCDPGQATKY LYELLYNDPI KIILMPGCSS VSTLVAEAAR MWNLIVLSYG , corresponding to amino acids 52-151 of Human GABA B Receptor 1
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... -
PurityAscites
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Purification notesPurified from ascites.
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ClonalityMonoclonal
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab55051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 108 kDa. | |
| IHC-FoFr | 1/1000. | |
| IHC-Fr | 1/1000. | |
| Flow Cyt | Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionReceptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites. -
Tissue specificityHighly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
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Sequence similaritiesBelongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
Contains 2 Sushi (CCP/SCR) domains. -
DomainAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
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Cellular localizationSecreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
- Information by UniProt
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Database links
- Entrez Gene: 2550 Human
- Entrez Gene: 54393 Mouse
- Entrez Gene: 81657 Rat
- Omim: 603540 Human
- SwissProt: Q9UBS5 Human
- SwissProt: Q9WV18 Mouse
- SwissProt: Q9Z0U4 Rat
- Unigene: 167017 Human
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Alternative names
- dJ271M21.1.1 antibody
- dJ271M21.1.2 antibody
- FLJ92613 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-GABA B Receptor 1 antibody (ab55051)This image is courtesy of an Abreview submitted by Carl Hobbsab55051 staining rat brain (hippocampus) sections by IHC-Fr. The sections were fixed with 4% paraformaldehyde in 0.3% PBS-Tween for 1h. Staining with ab5505 at a 1/1000 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 24h at 24°C. A donkey anti-rabbit Alexa568 polyclonal antibody at 1/1000 was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. GABA-B R1 expressed in the hippocampal neurons.
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Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)ICC/IF image of ab55051 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55051, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - Anti-GABA B Receptor 1 antibody (ab55051)GABA B Receptor 1 antibody (ab55051) at 1ug/lane + IMR-32 cell lysate at 25ug/lane. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-GABA B Receptor 1 antibody (ab55051)This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, FranceImmunohistochemistical detection of GABA B Receptor 1 antibody (ab55051) in PFA perfusion-fixed rat spinal cord section. ab55051 was used at 1/500, incubated for 18 hours @ 20°C in PBS-T 0.3%. Secondary antibody: Alexa Fluor® 546 anti-IgG Mouse (1/1000).
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Flow Cytometry - Anti-GABA B Receptor 1 antibody (ab55051)Overlay histogram showing SH-SY5Y cells stained with ab55051 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55051, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Jurcic N et al. GABAB receptors modulate Ca2+ but not G protein-gated inwardly rectifying K+ channels in cerebrospinal-fluid contacting neurones of mouse brainstem. J Physiol 597:631-651 (2019). Read more (PubMed: 30418666) »
- Feldmann M et al. Early Loss of Vision Results in Extensive Reorganization of Plasticity-Related Receptors and Alterations in Hippocampal Function That Extend Through Adulthood. Cereb Cortex 29:892-905 (2019). Read more (PubMed: 30535137) »
