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    gaba-b-receptor-1-antibody-ab55051.pdf

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Neuroscience Neurotransmitter Amino Acids GABA
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Anti-GABA B Receptor 1 antibody (ab55051)

  • Datasheet
Reviews (5)Q&A (3)References (38)

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Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)
  • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)
  • Western blot - Anti-GABA B Receptor 1 antibody (ab55051)
  • Flow Cytometry - Anti-GABA B Receptor 1 antibody (ab55051)
  • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)

Key features and details

  • Mouse monoclonal to GABA B Receptor 1
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-GABA B Receptor 1 antibody
    See all GABA B Receptor 1 primary antibodies
  • Description

    Mouse monoclonal to GABA B Receptor 1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment: AVYIGALFPM SGGWPGGQAC QPAVEMALED VNSRRDILPD YELKLIHHDS KCDPGQATKY LYELLYNDPI KIILMPGCSS VSTLVAEAAR MWNLIVLSYG , corresponding to amino acids 52-151 of Human GABA B Receptor 1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant on 29th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituents: 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride, 91% Water
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • GABA
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • GABA Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human GABA B Receptor 1 protein (ab112287)
  • Related Products

    • L-Glutamate, excitatory neurotransmitter (ab120049)
    • NMDA, excitotoxic amino acid (ab120052)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab55051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
Flow Cyt
Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
    Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
  • Tissue specificity

    Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    Contains 2 Sushi (CCP/SCR) domains.
  • Domain

    Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Target information above from: UniProt accession Q9UBS5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2550 Human
    • Omim: 603540 Human
    • SwissProt: Q9UBS5 Human
    • Unigene: 167017 Human
    • Alternative names

      • dJ271M21.1.1 antibody
      • dJ271M21.1.2 antibody
      • FLJ92613 antibody
      • GABA-B receptor 1 antibody
      • GABA-B-R1 antibody
      • GABA-BR1 antibody
      • GABAB R1 antibody
      • GABAB subunit 1c antibody
      • GABABR1 antibody
      • GABBR1 3 antibody
      • GABBR1 antibody
      • GABR1_HUMAN antibody
      • Gamma aminobutyric acid (GABA) B receptor 1 antibody
      • Gamma-aminobutyric acid type B receptor subunit 1 antibody
      • Gb1 antibody
      • GPRC3A antibody
      • Seven transmembrane helix receptor antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)
      Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)

      ICC/IF image of ab55051 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55051, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)
      Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)

      ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
      The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-GABA B Receptor 1 antibody (ab55051)
      Western blot - Anti-GABA B Receptor 1 antibody (ab55051)

      GABA B Receptor 1 antibody (ab55051) at 1ug/lane + IMR-32 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-GABA B Receptor 1 antibody (ab55051)
      Flow Cytometry - Anti-GABA B Receptor 1 antibody (ab55051)

      Overlay histogram showing SH-SY5Y cells stained with ab55051 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55051, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)
      Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 1 antibody (ab55051)

      ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
      The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

      This image was generated using the ascites version of the product.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (38)

    Publishing research using ab55051? Please let us know so that we can cite the reference in this datasheet.

    ab55051 has been referenced in 38 publications.

    • Wu Z  et al. Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia. Evid Based Complement Alternat Med 2020:3575231 (2020). PubMed: 32328126
    • Sarveazad A  et al. Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury. Stem Cell Res Ther 10:183 (2019). PubMed: 31234929
    • Dubovyk V & Manahan-Vaughan D Distinct Time-Course of Alterations of Groups I and II Metabotropic Glutamate Receptor and GABAergic Receptor Expression Along the Dorsoventral Hippocampal Axis in an Animal Model of Psychosis. Front Behav Neurosci 13:98 (2019). PubMed: 31139061
    • Feldmann M  et al. Early Loss of Vision Results in Extensive Reorganization of Plasticity-Related Receptors and Alterations in Hippocampal Function That Extend Through Adulthood. Cereb Cortex 29:892-905 (2019). PubMed: 30535137
    • Hudry E  et al. Opposing Roles of apolipoprotein E in aging and neurodegeneration. Life Sci Alliance 2:N/A (2019). PubMed: 30760557
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-GABA B Receptor 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (P90 Cerebellum)
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Loading amount
    100 µg
    Specification
    P90 Cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Estela Munoz

    Verified customer

    Submitted Dec 29 2017

    Western blot abreview for Anti-GABA B Receptor 1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Prefrontal cortex)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Prefrontal cortex
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2017

    Question

    Thank you so much for your response. We would like to take advantage of this program and order 3 antibodies. Just to clarify once we submit Abreview with our Flow cytometry data (positive or negative) we will get 3 antibodies for free? Is that correct?   We would be interested in getting the following antibodies:   Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html) Anti GABA B receptor 1 antibody: ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html)   Please let me know if there is a particular quote number we should use while ordering?   Thank you for the information.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test these products in Flow Cytometry. Each code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry for the product and include the related code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. You do not need to referencea quote number when ordering these products. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 26 2012

    Question

    Hello,   We are interested in ordering antibodies for the flow cytometry and I would like to make sure that antibody can get an access to the receptor subunits. Another requirement is that they need to react with human cells.   The antibodies we are interested are the following   Anti GABA A receptor alpha 1 subunit antibody  https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html Anti GABA A receptor beta 2 subunit antibody  https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html Anti GABA B receptor 1 antibody  https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html   Can you please verify that they will work with flow cytometry before I place an order?   Could you please also provide us a quote for the antibodies?   Thank in advance. We appreciate your help.   Best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for contacting us.

    I have had a chance to review each of the product which you have listed as well as our other products against the same targets. I would like to offer some recommendations.

    Anti GABA A receptor alpha 1 subunit antibody: ab33299 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab33299.html) and ab86320 (https://www.abcam.com/GABA-A-Receptor-alpha-1-antibody-ab86320.html) will both recognize the extracellular region of this protein in human tissue. Each will work as well as the other with the only difference being that ab86320 was created fromHuman GABA A Receptor alpha 1 andhas 100% homology, while ab33299 is created fromRat GABA A Receptor alpha 1with 93% homology.

    Anti GABA A receptor beta 2 subunit antibody: Ab42598 (https://www.abcam.com/GABA-A-Receptor-beta-2-antibody-ab42598.html)is raised against the extracellular region of this protein and will react with human tissues.

    Anti GABA B receptor 1 antibody: ab90883 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab90883.html) recognizes a cytoplasmic region of this protein and will require permeabilzation of the cells to be effective. Alternately ab55051 (https://www.abcam.com/GABA-B-Receptor-1-antibody-ab55051.html) recognizes the extracellular region of this protein. This will also work with human tissues.

    None of our antibodies against these targets have been tested in flow cytometry. As such I may offer you enrollment in our 100% Abreview Testing Discount. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please let me know if you have any questions or would like to take advantage of our testing discount with these products.


    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-GABA B Receptor 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Rat brain-Hippocampus)
    Specification
    Rat brain-Hippocampus
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% TritonX in 0.1% PBS
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Mar 02 2012

    Question

    After a few optimization process, I have attached pictures of WB with ab75239 (and ab55051 if it helps you) for abreview. In both cases, samples are the same (rat brain homogenates). We purchased ab55051 first and we were upset with so many bands. So, you kindly sent me ab75239 for abreview. With both Abs, there are 4-5 bands, including the ones around 95kDa & 130kDa. I think these bands are GABAb-R1B/C/D & -R1A/E since GABAb-Rs are supposed to be R1D (91kDa), R1B (95kDa), R1C (98kDa), and  & R1A (108kDa), R1E (12kDa). If I should change something or providew you additional information, please let me know. Thank you for your kindness again. PS: We are out of BCA Kit now and ordering it. So, we don't know the [protein] at all. Instead, I calculated wet weight of brain tissue before homogenization in RIPA buffer. Once BCA kit comes, I can give you the estimated [protein].  

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2011

    Answer

    Thanks so much for sending the results and for keeping me updated. I'm sorry to see that the antibody is still picking up so many bands, however you may be correct that some of the bands are isoforms of the target protein. In order for the Abreview to display on the datasheet, the information needs to be submitted at the "Submit an Abreview" link on our datasheet. This link will take you to a form with questions about your protocol, and you can also add the image to the review. If you have any questions about the review form, please let me know and I'd be happy to help. Let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-GABA B Receptor 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 30 min TBS + 0.5% Triton
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 18 2011

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-GABA B Receptor 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Dr. Karine Thibault

    Verified customer

    Submitted Oct 13 2010

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