Recombinant
RabMAb

Recombinant Anti-GABA B Receptor 1 antibody [EPR9404] (ab166604)

Overview

  • Product name

    Anti-GABA B Receptor 1 antibody [EPR9404]
    See all GABA B Receptor 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9404] to GABA B Receptor 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human GABA B Receptor 1 (UniProt ID: Q9UBS5).

  • Positive control

    • Fetal brain, U87-MG, HeLa and SH-SY5Y lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
      Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
    • Tissue specificity

      Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
      Contains 2 Sushi (CCP/SCR) domains.
    • Domain

      Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
    • Cellular localization

      Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • dJ271M21.1.1 antibody
      • dJ271M21.1.2 antibody
      • FLJ92613 antibody
      • GABA-B receptor 1 antibody
      • GABA-B-R1 antibody
      • GABA-BR1 antibody
      • GABAB R1 antibody
      • GABAB subunit 1c antibody
      • GABABR1 antibody
      • GABBR1 3 antibody
      • GABBR1 antibody
      • GABR1_HUMAN antibody
      • Gamma aminobutyric acid (GABA) B receptor 1 antibody
      • Gamma-aminobutyric acid type B receptor subunit 1 antibody
      • Gb1 antibody
      • GPRC3A antibody
      • Seven transmembrane helix receptor antibody
      see all

    Images

    • All lanes : Anti-GABA B Receptor 1 antibody [EPR9404] (ab166604) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : U87-MG lysate
      Lane 3 : HeLa lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 108 kDa
      Observed band size: 108 kDa

    References

    This product has been referenced in:

    • Li H  et al. Dihydromyricetin ameliorates memory impairment induced by acute sleep deprivation. Eur J Pharmacol 853:220-228 (2019). Read more (PubMed: 30876981) »
    • Hamilton KA  et al. Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus. PLoS One 13:e0196223 (2018). Read more (PubMed: 29715265) »
    See all 3 Publications for this product

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