Overview

  • Product name

    Anti-GABA B Receptor 1 antibody [N93A/49]
    See all GABA B Receptor 1 primary antibodies
  • Description

    Mouse monoclonal [N93A/49] to GABA B Receptor 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 873-977 of Rat GABA B Receptor 1

  • Positive control

    • WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S93A-49 to N93A/49, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab134314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).

1µg/ml of ab134314 was sufficient for detection of GABA B Receptor 1 in 20µg of rat brain membrane lysate, assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

ICC/IF 1/100.

Target

  • Function

    Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.
    Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
  • Tissue specificity

    Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    Contains 2 Sushi (CCP/SCR) domains.
  • Domain

    Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
  • Cellular localization

    Secreted and Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • dJ271M21.1.1 antibody
    • dJ271M21.1.2 antibody
    • FLJ92613 antibody
    • GABA-B receptor 1 antibody
    • GABA-B-R1 antibody
    • GABA-BR1 antibody
    • GABAB R1 antibody
    • GABAB subunit 1c antibody
    • GABABR1 antibody
    • GABBR1 3 antibody
    • GABBR1 antibody
    • GABR1_HUMAN antibody
    • Gamma aminobutyric acid (GABA) B receptor 1 antibody
    • Gamma-aminobutyric acid type B receptor subunit 1 antibody
    • Gb1 antibody
    • GPRC3A antibody
    • Seven transmembrane helix receptor antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for GABA B Receptor 1 (green) using ab134314 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes room temperature, 5 minutes room temperature.

    (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134314. (D) Composite.

  • Western blot detection of GABA B Receptor 1 in Rat brain membrane tissues using ab134314 at 1:1000 dilution.

References

ab134314 has not yet been referenced specifically in any publications.

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