Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] (ab68426)
Key features and details
- Rabbit monoclonal [EP2318Y] to GABA B Receptor 2/GABBR2 (phospho S893)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y]
See all GABA B Receptor 2/GABBR2 primary antibodies -
Description
Rabbit monoclonal [EP2318Y] to GABA B Receptor 2/GABBR2 (phospho S893) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain tissue. IHC-P: Human brain tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2318Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 105 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended. |
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 105 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended. |
Target
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Function
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. -
Tissue specificity
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. -
Domain
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 9568 Human
- Entrez Gene: 242425 Mouse
- Entrez Gene: 83633 Rat
- Omim: 607340 Human
- SwissProt: O75899 Human
- SwissProt: Q80T41 Mouse
- SwissProt: O88871 Rat
- Unigene: 198612 Human
see all -
Alternative names
- BcDNA:GH07312 antibody
- CG6706 antibody
- CT20836 antibody
see all
Images
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All lanes : Anti-GABA B Receptor 2/GABBR2 (phospho S893) antibody [EP2318Y] (ab68426) at 1/1000 dilution
Lane 1 : Human brain lysate - untreated
Lane 2 : Human brain lysate - treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Observed band size: 106 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of phosphorylated GABA B Receptor 2/GABBR2 expression on paraffin-embedded Human brain tissue using ab68426 at 1/100 dilution. Heat mediated antigen retrieval was performed, and an HRP-conjugated polymerized secondary antibody was used.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab68426 has been referenced in 1 publication.
- Kurata S et al. Exogenous gamma-aminobutyric acid addition enhances porcine sperm acrosome reaction. Anim Sci J 93:e13744 (2022). PubMed: 35699686