Recombinant
RabMAb

Recombinant Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (ab75838)

Overview

  • Product name

    Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y]
    See all GABA B Receptor 2/GABBR2 primary antibodies
  • Description

    Rabbit monoclonal [EP2411Y] to GABA B Receptor 2/GABBR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GABA B Receptor 2/GABBR2 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human cerebellum lysate; human brain tissue.
  • General notes

     

     

     This product was previously labelled as GABA B Receptor 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500. Predicted molecular weight: 106 kDa.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
    • Tissue specificity

      Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    • Domain

      Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BcDNA:GH07312 antibody
      • CG6706 antibody
      • CT20836 antibody
      • D Gaba2 antibody
      • FLJ36928 antibody
      • G protein coupled receptor 51 antibody
      • G-protein coupled receptor 51 antibody
      • GAB B R2 antibody
      • GABA B R2 antibody
      • GABA B receptor 2 antibody
      • GABA-B receptor 2 antibody
      • GABA-B-R2 antibody
      • GABA-BR2 antibody
      • GABAB R2 antibody
      • GABABR 2 antibody
      • GABABR2 antibody
      • GABB R2 antibody
      • GABBR 2 antibody
      • Gabbr2 antibody
      • GABR2_HUMAN antibody
      • Gamma aminobutyric acid B receptor 2 antibody
      • Gamma aminobutyric acid GABA B receptor 2 antibody
      • Gamma aminobutyric acid type B receptor subunit 2 antibody
      • Gamma-aminobutyric acid type B receptor subunit 2 antibody
      • Gb 2 antibody
      • Gb2 antibody
      • GH07312 antibody
      • GPR 51 antibody
      • GPR51 antibody
      • GPRC 3B antibody
      • GPRC3B antibody
      • HG 20 antibody
      • HG20 antibody
      • HRIHFB2099 antibody
      • Metabotropic GABA B receptor subtype 2 antibody
      • OTTHUMP00000021776 antibody
      • OTTHUMP00000063797 antibody
      • R2 SUBUNIT antibody
      see all

    Images

    • All lanes : Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (ab75838) at 1/1000 dilution

      Lane 1 : Human hippocampus lysates unboiled with 5% NFDM/TBST
      Lane 2 : Human hippocampus lysates boiled with 5% NFDM/TBST
      Lane 3 : Mouse brain lysates unboiled with 5% NFDM/TBST
      Lane 4 : Mouse brain lysates boiled with 5% NFDM/TBST
      Lane 5 : Rat brain lysates unboiled with 5% NFDM/TBST
      Lane 6 : Rat brain lysates boiled unboiled with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 106 kDa
      Observed band size: 106 kDa



      Exposure time:

      Lane 1 and 2: 140 seconds
      Lane 3 to 6: 180 seconds

      We recommend not to boil the samples after lysis to get desired WB results.

    • ab75838, at a 1/100 dilution, staining GABA B Receptor 2/GABBR2 in paraffin embedded human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] (ab75838) at 1/500 dilution + human cerebellum lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 106 kDa
      Observed band size: 106 kDa

    • Ab75838 staining GABA B Receptor 2/GABBR2 in Rat Postnatal (P15) Cerebellum by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/400 in PBS with 2% donkey serum) for 16 hours at 25°C. An Alexa Fluor® 488 conjugated Donkey anti-rabbit, polyclonal was used as the secondary antibody.

      IHC and confocal microscopy for GABA B Receptor 2/GABBR2 (GABAB2) in the postnatal rat cerebellum. (A, C, E, G) Negative control by omission of the primary antibody at 10, 20, 40 and 60X. (B-B’’, D-D’’, F-F’’, H-H’’) Immunolabeling for GABAB2 (green) and/or the calcium-binding protein calbindin (red) at 10, 20, 40 and 60X. (I-K) Zooms of the insets shown in F-F’’. Specific signal for GABAB2 is mainly observed in the molecular layer (ML) and internal granular layer (IGL) of the cerebellar grey matter. Purkinje cells (PkL) are enriched in GABAB2 especially at the surface of the dendritic trees (white arrowheads) oriented towards the ML. Golgi II cells (yellow arrowhead) located in the IGL are also positive for GABAB2. EGL: external granular layer. n: calbindin-positive nucleus of a Purkinje cell. Orange arrowhead: GABAB2-immunoreactive granular cell in the IGL. WM: white matter. An Olympus FV 1000 confocal microscope was used and images were processed with Adobe Photoshop CS4. Figure generated by Juan Vilchez Aruani and Estela Muñoz, IHEM, UNCuyo, CONICET, Mendoza, Argentina.

      See Abreview

    • Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75838, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:

    • Wang D  et al. Response of the maternal hypothalamus to cold stress during late pregnancy in rats. Brain Res 1722:146354 (2019). Read more (PubMed: 31356783) »
    • Sheilabi MA  et al. Quantitative expression and localization of GABAB receptor protein subunits in hippocampi from patients with refractory temporal lobe epilepsy. Neuropharmacology 136:117-128 (2018). Read more (PubMed: 28782512) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Adult cerebellum)
    Gel Running Conditions
    Reduced Denaturing (8% gel)
    Loading amount
    100 µg
    Specification
    Adult cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Estela Munoz

    Verified customer

    Submitted Nov 02 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Postnatal (P15) Cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH 6
    Permeabilization
    No
    Specification
    Postnatal (P15) Cerebellum
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Dr. Estela Munoz

    Verified customer

    Submitted Jul 05 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain section)
    Permeabilization
    Yes - 0.2% Triton-x100
    Specification
    brain section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 01 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: High pH
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    10% buffered formalin

    Abcam user community

    Verified customer

    Submitted Oct 23 2014

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