Recombinant
RabMAb

Recombinant Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] - BSA and Azide free (ab230136)

Overview

  • Product name

    Anti-GABA B Receptor 2/GABBR2 antibody [EP2411Y] - BSA and Azide free
    See all GABA B Receptor 2/GABBR2 primary antibodies
  • Description

    Rabbit monoclonal [EP2411Y] to GABA B Receptor 2/GABBR2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    (C terminal).

  • Positive control

    • Human cerebellum lysate; human brain tissue.
  • General notes

    ab230136 is the carrier-free version of ab75838 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab230136 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as GABA B Receptor 2

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 106 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
    • Tissue specificity

      Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
    • Domain

      Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • BcDNA:GH07312 antibody
      • CG6706 antibody
      • CT20836 antibody
      • D Gaba2 antibody
      • FLJ36928 antibody
      • G protein coupled receptor 51 antibody
      • G-protein coupled receptor 51 antibody
      • GAB B R2 antibody
      • GABA B R2 antibody
      • GABA B receptor 2 antibody
      • GABA-B receptor 2 antibody
      • GABA-B-R2 antibody
      • GABA-BR2 antibody
      • GABAB R2 antibody
      • GABABR 2 antibody
      • GABABR2 antibody
      • GABB R2 antibody
      • GABBR 2 antibody
      • Gabbr2 antibody
      • GABR2_HUMAN antibody
      • Gamma aminobutyric acid B receptor 2 antibody
      • Gamma aminobutyric acid GABA B receptor 2 antibody
      • Gamma aminobutyric acid type B receptor subunit 2 antibody
      • Gamma-aminobutyric acid type B receptor subunit 2 antibody
      • Gb 2 antibody
      • Gb2 antibody
      • GH07312 antibody
      • GPR 51 antibody
      • GPR51 antibody
      • GPRC 3B antibody
      • GPRC3B antibody
      • HG 20 antibody
      • HG20 antibody
      • HRIHFB2099 antibody
      • Metabotropic GABA B receptor subtype 2 antibody
      • OTTHUMP00000021776 antibody
      • OTTHUMP00000063797 antibody
      • R2 SUBUNIT antibody
      see all

    Images

    • This IHC data was generated using the same anti-GABA B Receptor 2/GABBR2 antibody clone, EP2411Y, in a different buffer formulation (cat# ab75838).

      Ab75838 staining GABA B Receptor 2/GABBR2 in Rat Postnatal (P15) Cerebellum by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/400 in PBS with 2% donkey serum) for 16 hours at 25°C. An Alexa Fluor® 488 conjugated Donkey anti-rabbit, polyclonal was used as the secondary antibody.

      IHC and confocal microscopy for GABA B Receptor 2/GABBR2 (GABAB2) in the postnatal rat cerebellum. (A, C, E, G) Negative control by omission of the primary antibody at 10, 20, 40 and 60X. (B-B’’, D-D’’, F-F’’, H-H’’) Immunolabeling for GABAB2 (green) and/or the calcium-binding protein calbindin (red) at 10, 20, 40 and 60X. (I-K) Zooms of the insets shown in F-F’’. Specific signal for GABAB2 is mainly observed in the molecular layer (ML) and internal granular layer (IGL) of the cerebellar grey matter. Purkinje cells (PkL) are enriched in GABAB2 especially at the surface of the dendritic trees (white arrowheads) oriented towards the ML. Golgi II cells (yellow arrowhead) located in the IGL are also positive for GABAB2. EGL: external granular layer. n: calbindin-positive nucleus of a Purkinje cell. Orange arrowhead: GABAB2-immunoreactive granular cell in the IGL. WM: white matter. An Olympus FV 1000 confocal microscope was used and images were processed with Adobe Photoshop CS4. Figure generated by Juan Vilchez Aruani and Estela Muñoz, IHEM, UNCuyo, CONICET, Mendoza, Argentina.

    • Immunofluorescence analysis of GABA B Receptor 2/GABBR2 using ab75838 at 1/400 dilution.
      Left panel = normal rat DRG neurons.
      Right panel = rat DRG neurons transfected with siRNAs to disrupt GABA B Receptor 2/GABBR2.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75838).

    • Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75838, 1/50 dilution) for 30 min at 22�C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22�C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75838).

    • This IHC data was generated using the same anti-GABA B Receptor 2/GABBR2 antibody clone, EP2411Y, in a different buffer formulation (cat# ab75838).

      ab75838, at a 1/100 dilution, staining GABA B Receptor 2/GABBR2 in paraffin embedded human brain tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab230136 has not yet been referenced specifically in any publications.

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