Recombinant Anti-GABA B Receptor 2/GABBR2 (phospho S884) antibody [EP2317Y] (ab68425)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GABA B Receptor 2/GABBR2 (phospho S884) antibody [EP2317Y]
See all GABA B Receptor 2/GABBR2 primary antibodies -
Description
Rabbit monoclonal [EP2317Y] to GABA B Receptor 2/GABBR2 (phospho S884) -
Host species
Rabbit -
Specificity
Detectes GABA B Receptor 2/GABBR2 only when phosphorylated at serine 884 -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
corresponding to Human GABA B Receptor 2/GABBR2.
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Positive control
- Brain
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as GABA B Receptor 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2317Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab68425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2500 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). | |
| IP | 1/30. |
Target
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Function
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. -
Tissue specificity
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. -
Domain
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 9568 Human
- Entrez Gene: 242425 Mouse
- Omim: 607340 Human
- SwissProt: O75899 Human
- SwissProt: Q80T41 Mouse
- Unigene: 198612 Human
- Unigene: 101909 Mouse
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Alternative names
- BcDNA:GH07312 antibody
- CG6706 antibody
- CT20836 antibody
see all
Images
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![Western blot - Anti-GABA B Receptor 2/GABBR2 (phospho S884) antibody [EP2317Y] (ab68425)](https://www.abcam.com/ps/products/68/ab68425/Images/GABA(B) R2_WB.gif)
Western blot - Anti-GABA B Receptor 2/GABBR2 (phospho S884) antibody [EP2317Y] (ab68425)All lanes : Anti-GABA B Receptor 2/GABBR2 (phospho S884) antibody [EP2317Y] (ab68425) at 1/5000 dilution
Lane 1 : Untreated Human brain lysate
Lane 2 : AP-treated Human brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Protocols
Datasheets and documents
References
ab68425 has not yet been referenced specifically in any publications.
