Recombinant
RabMAb

Recombinant Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)

Overview

  • Product name

    Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
    See all GABA Transporter 1 / GAT 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30531

  • Positive control

    • Fetal brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12998
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
    • Sequence similarities

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
    • Domain

      The PDZ domain-binding motif is involved in the interaction with MPP5.
    • Cellular localization

      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Information by UniProt
    • Database links

    • Alternative names

      • GABATHG antibody
      • GABATR antibody
      • GABT 1 antibody
      • GABT1 antibody
      • GAT-1 antibody
      • GAT1 antibody
      • SC6A1_HUMAN antibody
      • Slc6a1 antibody
      • Sodium and chloride dependent GABA transporter 1 antibody
      • Sodium- and chloride-dependent GABA transporter 1 antibody
      • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
      • Solute carrier family 6 member 1 antibody
      see all

    Images

    • All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution

      Lane 1 : Human brain lysates unboiled with 5% NFDM/TBST
      Lane 2 : Human brain lysates boiled with 5% NFDM/TBST
      Lane 3 : Mouse brain unboiled with 5% NFDM/TBST
      Lane 4 : Mouse brain boiled with 5% NFDM/TBST
      Lane 5 : Rat brain unboiled with 5% NFDM/TBST
      Lane 6 : Rat brain boiled with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 67 kDa
      Observed band size: 67 kDa



      Exposure time:

      Lane 1 to 2: 20 seconds
      Lane 3 to 6: 8 seconds

      We recommend not to boil the samples after lysis to get desired WB results.

    • Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg

      Predicted band size: 67 kDa

    References

    This product has been referenced in:

    • Ren W  et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol N/A:N/A (2018). Read more (PubMed: 30523310) »
    • Maolakuerban N  et al. MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther 19:282-291 (2018). Read more (PubMed: 29394133) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
    Specification
    Cryopreserved embryonic cortical neurons (QBMcells
    Permeabilization
    No
    Fixative
    paraformaldehyde with picric acid

    Ms. Babben Tinner

    Verified customer

    Submitted Mar 23 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Inner plexiform layer, outer plexiform lay)
    Specification
    Retina, Inner plexiform layer, outer plexiform lay
    Permeabilization
    Yes - triton X
    Fixative
    Paraformaldehyde

    Dr. Ryan Macdonald

    Verified customer

    Submitted Nov 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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