Product nameAnti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
See all GABA Transporter 1 / GAT 1 primary antibodies
DescriptionRabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P30531
- Fetal brain lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab177483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 67 kDa.|
FunctionTerminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
DomainThe PDZ domain-binding motif is involved in the interaction with MPP5.
Cellular localizationCell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
- Information by UniProt
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
All lanes : Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/10000 dilution
Lane 1 : Human brain lysates unboiled with 5% NFDM/TBST
Lane 2 : Human brain lysates boiled with 5% NFDM/TBST
Lane 3 : Mouse brain unboiled with 5% NFDM/TBST
Lane 4 : Mouse brain boiled with 5% NFDM/TBST
Lane 5 : Rat brain unboiled with 5% NFDM/TBST
Lane 6 : Rat brain boiled with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Lane 1 to 2: 20 seconds
Lane 3 to 6: 8 seconds
We recommend not to boil the samples after lysis to get desired WB results.
Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg
Predicted band size: 67 kDa
This product has been referenced in:
- Ren W et al. Slc6a13 deficiency promotes Th17 responses during intestinal bacterial infection. Mucosal Immunol N/A:N/A (2018). Read more (PubMed: 30523310) »
- Maolakuerban N et al. MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1. Cancer Biol Ther 19:282-291 (2018). Read more (PubMed: 29394133) »