Immunohistochemistry (Frozen sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Inner plexiform layer, outer plexiform lay)
Specification
Retina, Inner plexiform layer, outer plexiform lay
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA blocking Solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: invitrogen Alexafluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Very strong staining in the inner plexiform layer at 4dpf. Sodium Citrate antigen retrieval was used.

Dr. Ryan Macdonald

Verified customer

Submitted Nov 07 2014

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