Immunocytochemistry/ Immunofluorescence abreview for Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]

Inconclusive
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
Specification
Cryopreserved embryonic cortical neurons (QBMcells
Permeabilization
No
Fixative
paraformaldehyde with picric acid

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10mM PBS with 0.3% Triton-X
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
QBM Cell Science cryopreserved rat cortical neurons cultured for 7 days on poly-D-lysine coated 96 well plates. Inconclusive results. Possibly specific yet a NeuN-like immunofluorescence labeling also seen.
Abcam response
Many thanks for your valuable feedback. We welcome further customer comments around the specificity of this staining pattern

Ms. Babben Tinner

Verified customer

Submitted Mar 23 2015

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