RecombinantRabMAb

Recombinant Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
  • Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
  • Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24202-20] to GABA Transporter 1 / GAT 1
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20]
    See all GABA Transporter 1 / GAT 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24202-20] to GABA Transporter 1 / GAT 1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse GABA Transporter 1/ GAT 1 aa 1-100. The exact sequence is proprietary.
    Database link: P31648

  • Positive control

    • IHC-P: Mouse cerebrum and retina tissue; Rat cerebrum and retina tissue. IHC-Fr: Mouse retina tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24202-20

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab259971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/50.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IP or WB.

    • Target

    • Function

      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

    • Sequence similarities

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.

    • Domain

      The PDZ domain-binding motif is involved in the interaction with MPP5.

    • Cellular localization

      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Information by UniProt

    • Database links

      • Alternative names

        • GABATHG antibody
        • GABATR antibody
        • GABT 1 antibody
        • GABT1 antibody
        • GAT-1 antibody
        • GAT1 antibody
        • SC6A1_HUMAN antibody
        • Slc6a1 antibody
        • Sodium and chloride dependent GABA transporter 1 antibody
        • Sodium- and chloride-dependent GABA transporter 1 antibody
        • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
        • Solute carrier family 6 member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

        Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 dilution (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 21382352). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/50 (9.82 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

        Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID: 2387399). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

        Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse retina (PMID: 18975268).The section was incubated with ab259971 for 30 mins at room temperature .The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

        Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining on rat retina (PMID: 2387399).The section was incubated with ab259971 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)
        Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] (ab259971)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab259971? Please let us know so that we can cite the reference in this datasheet.

      ab259971 has not yet been referenced specifically in any publications.

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