RecombinantRabMAb

Recombinant Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
  • Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
  • Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24202-20] to GABA Transporter 1 / GAT 1 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free
    See all GABA Transporter 1 / GAT 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24202-20] to GABA Transporter 1 / GAT 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse GABA Transporter 1/ GAT 1 aa 1-100. The exact sequence is proprietary.
    Database link: P31648

  • Positive control

    • IHC-P: Mouse cerebrum and retina tissue; Rat cerebrum and retina tissue. IHC-Fr: Mouse retina tissue.

  • General notes

    ab277642 is the carrier-free version of ab259971. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab277642 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab277642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IP or WB.

    • Target

    • Function

      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

    • Sequence similarities

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.

    • Domain

      The PDZ domain-binding motif is involved in the interaction with MPP5.

    • Cellular localization

      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Information by UniProt

    • Database links

      • Alternative names

        • GABATHG antibody
        • GABATR antibody
        • GABT 1 antibody
        • GABT1 antibody
        • GAT-1 antibody
        • GAT1 antibody
        • SC6A1_HUMAN antibody
        • Slc6a1 antibody
        • Sodium and chloride dependent GABA transporter 1 antibody
        • Sodium- and chloride-dependent GABA transporter 1 antibody
        • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
        • Solute carrier family 6 member 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

        This data was developed using ab259971, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 dilution (0.982 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum (PMID: 21382352). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

        This data was developed using ab259971, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/50 (9.82 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse retina is observed. The nuclear counterstain was DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

        This data was developed using ab259971, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum (PMID: 2387399). The section was incubated with ab259971 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

        This data was developed using ab259971, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse retina (PMID: 18975268).The section was incubated with ab259971 for 30 mins at room temperature .The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

        This data was developed using ab259971, the same antibody clone in a different buffer formulation.

        Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling GABA Transporter 1 / GAT 1 with ab259971 at 1/500 (0.982 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) . Positive staining on rat retina (PMID: 2387399).The section was incubated with ab259971 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      • Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)
        Anti-GABA Transporter 1 / GAT 1 antibody [EPR24202-20] - BSA and Azide free (ab277642)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab277642? Please let us know so that we can cite the reference in this datasheet.

      ab277642 has not yet been referenced specifically in any publications.

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