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Anti-GABA Transporter 1 / GAT 1 antibody (ab426)

  • Datasheet
Reviews (3)Q&A (10)References (24)

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Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)

    Key features and details

    • Rabbit polyclonal to GABA Transporter 1 / GAT 1
    • Suitable for: WB
    • Reacts with: Rat, Chinese hamster
    • Isotype: IgG

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    Overview

    • Product name

      Anti-GABA Transporter 1 / GAT 1 antibody
      See all GABA Transporter 1 / GAT 1 primary antibodies
    • Description

      Rabbit polyclonal to GABA Transporter 1 / GAT 1
    • Host species

      Rabbit
    • Specificity

      Reacts specifically with a 72 kDa protein.
    • Tested Applications & Species

      Application Species
      WB
      Chinese hamster
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
      Database link: P23978

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituent: Whole serum
    • Concentration information loading...
    • Purity

      Whole antiserum
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Amino Acids
      • GABA
      • Neuroscience
      • Neurotransmitter
      • Transporters
      • GABA

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab426 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Chinese hamster
    All applications
    Human
    Application Abreviews Notes
    WB (1)
    1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
    Notes
    WB
    1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).

    Target

    • Function

      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
    • Sequence similarities

      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
    • Domain

      The PDZ domain-binding motif is involved in the interaction with MPP5.
    • Cellular localization

      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Target information above from: UniProt accession P30531 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6529 Human
      • Entrez Gene: 79212 Rat
      • Omim: 137165 Human
      • SwissProt: P30531 Human
      • SwissProt: P23978 Rat
      • Unigene: 443874 Human
      • Unigene: 10035 Rat
      • Alternative names

        • GABATHG antibody
        • GABATR antibody
        • GABT 1 antibody
        • GABT1 antibody
        • GAT-1 antibody
        • GAT1 antibody
        • SC6A1_HUMAN antibody
        • Slc6a1 antibody
        • Sodium and chloride dependent GABA transporter 1 antibody
        • Sodium- and chloride-dependent GABA transporter 1 antibody
        • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
        • Solute carrier family 6 member 1 antibody
        see all

      Images

      • Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
        Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
        All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

        Lane 1 : Rat Hippocampus Tissue Lysate
        Lane 2 : Spinal Cord (Rat) Tissue Lysate
        Lane 3 : Brain (Rat) Tissue Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 67 kDa
        Observed band size: 74 kDa
        why is the actual band size different from the predicted?


        Exposure time: 30 seconds


        GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (24)

      Publishing research using ab426? Please let us know so that we can cite the reference in this datasheet.

      ab426 has been referenced in 24 publications.

      • Guimarães-Souza EM  et al. Retinal alterations in a pre-clinical model of an autism spectrum disorder. Mol Autism 10:19 (2019). PubMed: 31011411
      • Zhang W  et al. Role of microRNA-155 in modifying neuroinflammation and ?-aminobutyric acid transporters in specific central regions after post-ischaemic seizures. J Cell Mol Med 23:5017-5024 (2019). PubMed: 31144434
      • Leonetti A  et al. Epileptic seizures and oxidative stress in a mouse model over-expressing spermine oxidase. Amino Acids N/A:N/A (2019). PubMed: 31197571
      • Ge MM  et al. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain. Eur J Pharmacol 858:172475 (2019). PubMed: 31228456
      • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). PubMed: 29693164
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 13 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Guinea pig Tissue sections (Brain_Region B)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
      Permeabilization
      No
      Specification
      Brain_Region B
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
      Fixative
      formalin
      Read More

      Poonam Rani

      Verified customer

      Submitted Apr 07 2016

      Question

      wir benötigen Primär-Antikörper, die für Elektronenmikroskopie (TEM) am Gehirn der Maus geeignet sind (postembedding immunogold).

      Konkret handelt es sich um folgende nachzuweisende Proteine:
      GABA A-Rezeptor
      GABA B-Rezeptor
      GABA-Transporter (GAT 1 + GAT3)

      Glycin -Rezeptor
      Glycin-Transporter (GlyT1 + GlyT2)

      Welche Antikörper aus Ihrem Sortiment können Sie uns für unsere Anwendung empfehlen.

      Read More

      Abcam community

      Verified customer

      Asked on May 30 2012

      Answer

      Vielen Dank für Ihre Anfrage.

      Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstellen.
      Desweiteren gibt es mehrere Subtypen der GABA A- und B-Rezeptoren sowie des Glycin -Rezeptors. Haben Sie da eine spezielle Vorliebe?

      Ich freue mich auf Ihre Antwort.

      Read More

      Abcam Scientific Support

      Answered on May 30 2012

      Question

      Thank you for your fast respond. This time we would like to get a REFUND. This is the best option for us. When we will be ready we'll buy more antibodies in Abcam.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 04 2012

      Answer

      Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

      Read More

      Abcam Scientific Support

      Answered on Apr 04 2012

      Question

      Here are answers on your questions about ab72448 using in our WB studies:


      Could you tell me the lysis buffer that you have used? RIPA + proteinase inhibitor + PMSF
      Have you reduced and denatured your samples? Which method/buffers did you use?
      Invitrogen 4x Loading buffer + 10x Reducing agent. Samples were heated for 5 min at 85C.
      How much total protein did you use per well? 10 µg/well
      Did you load a loading control? Which LC did you use? What were the results?
      Loading control – GAPDH, very nice bends
      Did you load a positive control? Which PC did you use? What were the results?
      Positive control: parietal cortex, rat. The primary Abs: ab426 (Abcam) and anti-Gat-1 (Alomone Labs) shows a strong bend for GAT-1 with concentration of protein 10 µg/well from the positive control. But ab72448 (Abcam) did not show the clear bend for GAT-1 (only very weak signal between 55 and 58 kDa, but not 67kDa as was stated in description for this product).
      What membrane type did you transfer to? PVDF membrane
      What blocking steps did you use? 5% NF dry milk /TBST
      What dilutions of this product did you use? How long and at what temperature was this antibody incubated? Ab72448 (dilution 1:500), overnight at +4C.
      What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated? Odyssey (1 hr), goat anti-Rabbit Fluorescent Ab (IRDye680) 1:4000; HRP (1.5 hrs), donkey anti-rabbit HRP (1:4000)
      Have you run a no primary antibody control blot? What were the results? We did not.
      What is the wash buffer that you use? TBST
      Could you provide the Abcam with any images you may have? Yes (on the image: first well (to the right from the ladder) is positive control (parietal cortex, rat), the others are hypothalamic tissue, rat. Loading Control - GAPDH (green).

      Read More

      Abcam community

      Verified customer

      Asked on Apr 02 2012

      Answer

      Thank you for your message and for providing this further information.

      From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement product, refund or credit note in compensation.

      I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Apr 02 2012

      Question

      Called to discuss problems using ab72448.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 30 2012

      Answer

      Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      As we discussed on the phone, I am including a brief questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

      I look forward to receiving your reply.



      Could you tell me the lysis buffer that you have used?

      Have you reduced and denatured your samples? Which method/buffers did you use?

      How much total protein did you use per well?

      Did you load a loading control? Which LC did you use? What were the results?

      Did you load a positive control? Which PC did you use? What were the results?

      What membrane type did you transfer to?

      What blocking steps did you use?

      What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

      What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

      Have you run a no primary antibody control blot? What were the results?

      What is the wash buffer that you use?

      Could you provide the Abcam with any images you may have?

      Finally, would you be able to provide the order confirmation number or PO number used to purchase this?

      Read More

      Abcam Scientific Support

      Answered on Mar 30 2012

      Question

      Hello, The product codes (catalog number) are the follows:   GABA transporter 1/GAT 1: ab426   GABA A Receptor alpha 2: ab72445   I would be very appreciate if you could provide me the concentrations of these antibodies. Thank you very much, Regards,      

      Read More

      Abcam community

      Verified customer

      Asked on Dec 01 2011

      Answer

      Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. However, as an estimate, serum is thought to contain about 1 - 10 mg/ml of antibody. With regards to ab72445, the concentration of this antibody is likely to be lot specific. I woudl appreciate if you could confirm the lot number you have, and I can request the concentration for you. I hope this will be helpful, and I look forward to hearing from you.

      Read More

      Abcam Scientific Support

      Answered on Dec 01 2011

      Western blot abreview for Anti-GABA Transporter 1 / GAT 1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (brain)
      Loading amount
      10 µg
      Specification
      brain
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
      Read More

      Mrs. Olga Cravetchi

      Verified customer

      Submitted Nov 22 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (mouse retina sections)
      Specification
      mouse retina sections
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Mr. Kevin Jin

      Verified customer

      Submitted Nov 19 2010

      Question

      PRE-SALES ENQUIRY Concerning ab426, the rabbit anti-GAT1 antibody, has this been shown to work in immunocytochemistry/immunofluorescence of primary neurons? And if so, what dilution and in what conditions? Formaldehyde fixed with glutaraldehyde or just formaldehyde-fixed samples? Thanks.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 30 2004

      Answer

      Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded tissue sections. We have also received a good review stating that ab426 worked nicely on embryionic chick ciliary ganglion. Please take a look at the review section on the datasheet (top panel). If the primary neurons express the target protein, you can use this antibody for IF/ICC. We would suggest fixing the cells in acetone or ethanol rather than in formalin. Try this antibody at 1:100 and adjust accordingly from the results obtained. If you need anything further or any help then please let us know.

      Read More

      Abcam Scientific Support

      Answered on Sep 01 2004

      Question

      today we received your package. Unfortunately there were only a cup, some ice and some papers in, but no antibody. Please could you send us the antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 23 2004

      Answer

      Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and will send it to you as soon as possible. Unfortunately, to your dismay, the packaging still got shipped. Sorry for the inconvenience.

      Read More

      Abcam Scientific Support

      Answered on Mar 24 2004

      1-10 of 13 Abreviews or Q&A

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