Anti-GABA Transporter 1 / GAT 1 antibody (ab426)

Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstellen.
Desweiteren gibt es mehrere Subtypen der GABA A- und B-Rezeptoren sowie des Glycin -Rezeptors. Haben Sie da eine spezielle Vorliebe?

Ich freue mich auf Ihre Antwort.

Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the time you have spent on these experiments and would be pleased to arrange a replacement product, refund or credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I am including a brief questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.



Could you tell me the lysis buffer that you have used?

Have you reduced and denatured your samples? Which method/buffers did you use?

How much total protein did you use per well?

Did you load a loading control? Which LC did you use? What were the results?

Did you load a positive control? Which PC did you use? What were the results?

What membrane type did you transfer to?

What blocking steps did you use?

What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

Have you run a no primary antibody control blot? What were the results?

What is the wash buffer that you use?

Could you provide the Abcam with any images you may have?

Finally, would you be able to provide the order confirmation number or PO number used to purchase this?

Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. However, as an estimate, serum is thought to contain about 1 - 10 mg/ml of antibody. With regards to ab72445, the concentration of this antibody is likely to be lot specific. I woudl appreciate if you could confirm the lot number you have, and I can request the concentration for you. I hope this will be helpful, and I look forward to hearing from you.

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded tissue sections. We have also received a good review stating that ab426 worked nicely on embryionic chick ciliary ganglion. Please take a look at the review section on the datasheet (top panel). If the primary neurons express the target protein, you can use this antibody for IF/ICC. We would suggest fixing the cells in acetone or ethanol rather than in formalin. Try this antibody at 1:100 and adjust accordingly from the results obtained. If you need anything further or any help then please let us know.

Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and will send it to you as soon as possible. Unfortunately, to your dismay, the packaging still got shipped. Sorry for the inconvenience.