Anti-GABA Transporter 1 / GAT 1 antibody (ab426)

Abreviews (3)Q&A (10)Specific References (15)

Overview

  • Product name
    Anti-GABA Transporter 1 / GAT 1 antibody
    See all GABA Transporter 1 / GAT 1 primary antibodies
  • Description
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • Host species
    Rabbit
  • Specificity
    Reacts specifically with a 72 kDa protein.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
    Database link: P23978

  • Positive control
    • IHC-Fr: Mouse retinal tissue. WB: Rat hippocampus, spinal cord and brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Sequence similarities
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • Domain
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • Cellular localization
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
    Immunohistochemistry (Frozen sections) - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)This image is courtesy of an Abreview submitted by Kevin Jin
    ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
    Western blot - Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
    All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 74 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:
  • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). Read more (PubMed: 29693164) »
  • Ding XD  et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »

See all 15 Publications for this product

Publishing research using ab426? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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13 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

 Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin
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Username

Poonam Rani

Verified customer

Submitted Apr 07 2016

wir benötigen Primär-Antikörper, die für Elektronenmikroskopie (TEM) am Gehirn der Maus geeignet sind (postembedding immunogold).

Konkret handelt es sich um folgende nachzuweisende Proteine:
GABA A-Rezeptor
GABA B-Rezeptor
GAB...

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Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstelle...

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Thank you for your fast respond. This time we would like to get a REFUND. This is the best option for us. When we will be ready we'll buy more antibodies in Abcam.

Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchas...

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Here are answers on your questions about ab72448 using in our WB studies:


Could you tell me the lysis buffer that you have used? RIPA + proteinase inhibitor + PMSF
Have you reduced and denatured your samples? Which method/buffers did ...

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Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the ...

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Called to discuss problems using ab72448.

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

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Hello, The product codes (catalog number) are the follows:   GABA transporter 1/GAT 1: ab426   GABA A Receptor alpha 2: ab72445   I would be very appreciate if you could provide me the concentrations of these antibodies. Thank you very much, Regards,

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Thank you for your reply. As ab426 sold as whole antiserum, the concentration will not be known. Antibody concentration is usually measured using a protein assay. As serum will contain many proteins, this would not provide an accurate result. H...

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Western blot abreview for Anti-GABA Transporter 1 / GAT 1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Username

Mrs. Olga Cravetchi

Verified customer

Submitted Nov 22 2011

Immunohistochemistry (Frozen sections) abreview for Anti-GABA Transporter 1 / GAT 1 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Mr. Kevin Jin

Verified customer

Submitted Nov 19 2010

PRE-SALES ENQUIRY Concerning ab426, the rabbit anti-GAT1 antibody, has this been shown to work in immunocytochemistry/immunofluorescence of primary neurons? And if so, what dilution and in what conditions? Formaldehyde fixed with glutaraldehyde or j...

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Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody (ab426:Rabbit polyclonal to GABA Transporter 1 / GAT 1) has been tested and characterised for IHC both on frozen and formalin-fixed embedded ti...

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today we received your package. Unfortunately there were only a cup, some ice and some papers in, but no antibody. Please could you send us the antibody?

Thank you for your email. Concerning ab426, we received instructions from our technical department not to send you the vial which we have in stock currently, but to wait for a specific vial from our suppliers. We are still waiting for this vial and wi...

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