Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
Key features and details
- Rabbit polyclonal to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Rat, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-GABA Transporter 1 / GAT 1 antibody
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit polyclonal to GABA Transporter 1 / GAT 1 -
Host species
Rabbit -
Specificity
Reacts specifically with a 72 kDa protein. -
Tested Applications & Species
Application Species WB Chinese hamster -
Immunogen
Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
Database link: P23978
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Chinese hamster
|
All applications |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). |
Target
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Function
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPP5. -
Cellular localization
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
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Database links
- Entrez Gene: 6529 Human
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P23978 Rat
- Unigene: 443874 Human
- Unigene: 10035 Rat
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Alternative names
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
Images
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All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (24)
ab426 has been referenced in 24 publications.
- Guimarães-Souza EM et al. Retinal alterations in a pre-clinical model of an autism spectrum disorder. Mol Autism 10:19 (2019). PubMed: 31011411
- Zhang W et al. Role of microRNA-155 in modifying neuroinflammation and ?-aminobutyric acid transporters in specific central regions after post-ischaemic seizures. J Cell Mol Med 23:5017-5024 (2019). PubMed: 31144434
- Leonetti A et al. Epileptic seizures and oxidative stress in a mouse model over-expressing spermine oxidase. Amino Acids N/A:N/A (2019). PubMed: 31197571
- Ge MM et al. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain. Eur J Pharmacol 858:172475 (2019). PubMed: 31228456
- Qian Z et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). PubMed: 29693164