Anti-GABA Transporter 1 / GAT 1 antibody (ab426)
Key features and details
- Rabbit polyclonal to GABA Transporter 1 / GAT 1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-GABA Transporter 1 / GAT 1 antibody
See all GABA Transporter 1 / GAT 1 primary antibodies -
Description
Rabbit polyclonal to GABA Transporter 1 / GAT 1 -
Host species
Rabbit -
Specificity
Reacts specifically with a 72 kDa protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human -
Immunogen
Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
Database link: P23978 -
Positive control
- WB: Rat Hippocampus Tissue Lysate; Rat Spinal Cord Tissue Lysate; Rat Brain Tissue Lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
|
Notes |
---|
WB
1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa). |
Target
-
Function
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPP5. -
Cellular localization
Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons. - Information by UniProt
-
Database links
- Entrez Gene: 6529 Human
- Entrez Gene: 79212 Rat
- Omim: 137165 Human
- SwissProt: P30531 Human
- SwissProt: P23978 Rat
- Unigene: 443874 Human
- Unigene: 10035 Rat
-
Alternative names
- GABATHG antibody
- GABATR antibody
- GABT 1 antibody
see all
Images
-
All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
-
Datasheet download
References (36)
ab426 has been referenced in 36 publications.
- Zhang D et al. Identification of sodium homeostasis genes in Camelus bactrianus by whole transcriptome sequencing. FEBS Open Bio 12:864-876 (2022). PubMed: 35147292
- Lin Y et al. Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery. Theranostics 11:3760-3780 (2021). PubMed: 33664860
- Wang S et al. Decreased GABAergic signaling, fewer parvalbumin-, somatostatin- and calretinin-positive neurons in brain of a rat model of simulated transport stress. Res Vet Sci 134:86-95 (2021). PubMed: 33360121
- Chen M et al. Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models. Mol Neurodegener 16:14 (2021). PubMed: 33663578
- Balzano T et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage. Biomedicines 9:N/A (2021). PubMed: 34440206