Overview

  • Product name
    Anti-GABA Transporter 1 / GAT 1 antibody
    See all GABA Transporter 1 / GAT 1 primary antibodies
  • Description
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • Host species
    Rabbit
  • Specificity
    Reacts specifically with a 72 kDa protein.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Rat GABA Transporter 1/ GAT 1 (C terminal). 20 amino acids.
    Database link: P23978

  • Positive control
    • IHC-Fr: Mouse retinal tissue. WB: Rat hippocampus, spinal cord and brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 67 kDa).
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Sequence similarities
    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • Domain
    The PDZ domain-binding motif is involved in the interaction with MPP5.
  • Cellular localization
    Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Images

  • ab426 staining the GABA transporter 1 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2000 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-GABA Transporter 1 / GAT 1 antibody (ab426) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate
    Lane 2 : Spinal Cord (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    GABA Transporter 1/ GAT 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:
  • Qian Z  et al. Expression and functions of glutamate and ?-aminobutyric acid transporters in ischemic models. Mol Med Rep 17:8196-8202 (2018). Read more (PubMed: 29693164) »
  • Ding XD  et al. Impact of Botox-A SNAP-25 protein expression and the mechanism of inhibitory neurotransmitter imbalance in chronic sciatic nerve pain rat model. Exp Ther Med 13:2783-2786 (2017). Read more (PubMed: 28588664) »
See all 17 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin

Poonam Rani

Verified customer

Submitted Apr 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
10 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Mrs. Olga Cravetchi

Verified customer

Submitted Nov 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Mr. Kevin Jin

Verified customer

Submitted Nov 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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