Recombinant
RabMAb

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483)

Overview

  • Product name
    Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]
    See all GABA Transporter 1 / GAT 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12998] to GABA Transporter 1 / GAT 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P30531

  • Positive control
    • Fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12998
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 67 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
    • Sequence similarities
      Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
    • Domain
      The PDZ domain-binding motif is involved in the interaction with MPP5.
    • Cellular localization
      Cell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • GABATHG antibody
      • GABATR antibody
      • GABT 1 antibody
      • GABT1 antibody
      • GAT-1 antibody
      • GAT1 antibody
      • SC6A1_HUMAN antibody
      • Slc6a1 antibody
      • Sodium and chloride dependent GABA transporter 1 antibody
      • Sodium- and chloride-dependent GABA transporter 1 antibody
      • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
      • Solute carrier family 6 member 1 antibody
      see all

    Images

    • Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (ab177483) at 1/1000 dilution + Fetal brain lysate at 10 µg

      Predicted band size: 67 kDa

    References

    ab177483 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
    Specification
    Cryopreserved embryonic cortical neurons (QBMcells
    Permeabilization
    No
    Fixative
    paraformaldehyde with picric acid
    Username

    Ms. Babben Tinner

    Verified customer

    Submitted Mar 23 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Inner plexiform layer, outer plexiform lay)
    Specification
    Retina, Inner plexiform layer, outer plexiform lay
    Permeabilization
    Yes - triton X
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Nov 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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