Anti-GABA Transporter 2 antibody (ab229815)
Key features and details
- Rabbit polyclonal to GABA Transporter 2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GABA Transporter 2 antibody -
Description
Rabbit polyclonal to GABA Transporter 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human GABA Transporter 2 aa 1-100.
Database link: Q9NSD5 -
Positive control
- WB: Mouse heart, brain and kidney lysates. IHC-P: Human testis and kidney tissues. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 68 kDa).
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ICC/IF |
1/50 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 68 kDa). |
ICC/IF
1/50 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Sodium-dependent GABA and taurine transporter. In presynaptic terminals, regulates GABA signaling termination through GABA uptake. May also be involved in beta-alanine transport. -
Tissue specificity
Expressed in brain, kidney, lung, liver and testis. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A13 subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 102141142 Cynomolgus monkey
- Entrez Gene: 6540 Human
- Entrez Gene: 14412 Mouse
- SwissProt: Q2PG55 Cynomolgus monkey
- SwissProt: Q8TCC2 Human
- SwissProt: Q9NSD5 Human
- SwissProt: P31649 Mouse
- Unigene: 504398 Human
see all -
Alternative names
- GABA transport protein antibody
- GAT 2 antibody
- GAT-2 antibody
see all
Images
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All lanes : Anti-GABA Transporter 2 antibody (ab229815) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human testis tissue stained for GABA Transporter 2 using ab229815 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for GABA Transporter 2 using ab229815 at 1/100 dilution in immunohistochemical analysis.
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A549 (human lung carcinoma cell line) cells stained for GABA Transporter 2 (green) using ab229815 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229815 has been referenced in 1 publication.
- Stokum JA et al. A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain. J Cereb Blood Flow Metab 41:2546-2560 (2021). PubMed: 33818185