Key features and details
- Rabbit polyclonal to GABA Transporter 2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GABA Transporter 2 antibody
DescriptionRabbit polyclonal to GABA Transporter 2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey
- WB: Mouse heart, brain and kidney lysates. IHC-P: Human testis and kidney tissues. ICC/IF: A549 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab229815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 68 kDa).|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionSodium-dependent GABA and taurine transporter. In presynaptic terminals, regulates GABA signaling termination through GABA uptake. May also be involved in beta-alanine transport.
Tissue specificityExpressed in brain, kidney, lung, liver and testis.
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A13 subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- GABA transport protein antibody
- GAT 2 antibody
- GAT-2 antibody
All lanes : Anti-GABA Transporter 2 antibody (ab229815) at 1/500 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse kidney lysate
All lanes : Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 68 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Paraffin-embedded human testis tissue stained for GABA Transporter 2 using ab229815 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for GABA Transporter 2 using ab229815 at 1/100 dilution in immunohistochemical analysis.
A549 (human lung carcinoma cell line) cells stained for GABA Transporter 2 (green) using ab229815 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229815 has not yet been referenced specifically in any publications.