Overview

  • Product name
    Anti-GABA Transporter 3 / GAT 3 antibody
    See all GABA Transporter 3 / GAT 3 primary antibodies
  • Description
    Rabbit polyclonal to GABA Transporter 3 / GAT 3
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with a 74 kDa protein from CNS samples.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Chicken
    Predicted to work with: Mouse, Human
  • Immunogen

    15 residue C-terminal synthetic peptide (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/200 - 1/5000.

Target

References

This product has been referenced in:
  • Barca-Mayo O  et al. Astrocyte deletion of Bmal1 alters daily locomotor activity and cognitive functions via GABA signalling. Nat Commun 8:14336 (2017). IF . Read more (PubMed: 28186121) »
  • Zhang Y  et al. Astrocytic Process Plasticity and IKKß/NF-?B in Central Control of Blood Glucose, Blood Pressure, and Body Weight. Cell Metab 25:1091-1102.e4 (2017). Read more (PubMed: 28467927) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrtae buffer ph 6
Permeabilization
No
Specification
Brain_Region B
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
formalin

Abcam user community

Verified customer

Submitted Apr 07 2016

Answer

Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstellen.
Desweiteren gibt es mehrere Subtypen der GABA A- und B-Rezeptoren sowie des Glycin -Rezeptors. Haben Sie da eine spezielle Vorliebe?

Ich freue mich auf Ihre Antwort.

Read More

Answer

Thank you for contacting us.

The GAT3 antibody ab431 detected a band at 74 kDa in CNS samples, as noted on the datasheet. The precursor of the protein, before any post-translation modifications has a predicted molecular weight of about 70 kDa. Glycosylation of the GAT3 is presumed to cause the protein to run in the gel to 74 kDa.

Your result, detection at 100 kDa and 20 and 50, suggests the antibody is detecting something other than GAT3. Degradation of the sample might explain the bands at 20 and 50 kDa, if you are not using protesase inhibitors in your sample lysis buffer, but I have no explanation for the 100 kDa band.

In your reply, could you please include an image of the blot, and a few details of your protocol, such as how much sample you loaded per lane of the gel, the blocking buffer, and how much antibody was applied to the blot? If I cannot suggest a modification to your protocol, it may be best to replace the antibody with another, which I can offer free-of-charge.

For the GAT2 antibody, ab2896, the precursor is expected to run at 68 kDa. There are several sites on the GAT2 protein that are glycosylated, so it will likely run on the gel to somewhere between 68 and 75 kDa, assuming there are no other post-translation modifications.

Read More

Answer

These antibodies will work on both applications : frozen sections and paraffin embedded sections.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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