Overview

  • Product name
    Anti-GABARAP antibody
    See all GABARAP primary antibodies
  • Description
    Rabbit polyclonal to GABARAP
  • Host species
    Rabbit
  • Specificity
    This antibody recognizes human GABARAP (14 kD).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RSEGEKIRKKYPDRVPV

    , corresponding to amino acids 15-31 of Human GABARAP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab1398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Western Blot: use at 1:500-1:1,000 dilution.
    ELISA: use at 1:1,000-1:5,000 dilution

    • Target

    • Function
      Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • Tissue specificity
      Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the ATG8 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • Cellular localization
      Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • Database links
      • Alternative names
        • ATG8A antibody
        • FLC 3B antibody
        • FLC3B antibody
        • FLJ25768 antibody
        • GABA type A receptor associated protein antibody
        • GABA(A) receptor associated protein antibody
        • GABA(A) receptor-associated protein antibody
        • GABARAP a antibody
        • GABARAP antibody
        • Gamma aminobutyric acid receptor associated protein antibody
        • Gamma-aminobutyric acid receptor-associated protein antibody
        • GBRAP_HUMAN antibody
        • MGC120154 antibody
        • MGC120155 antibody
        • MM 46 antibody
        • MM46 antibody
        see all

      Images

      References

      This product has been referenced in:
      • Li L  et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
      • Belichenko PV  et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . Read more (PubMed: 19034952) »

      See all 2 Publications for this product

      Publishing research using ab1398? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      4 reviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-GABARAP antibody

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Macrophages)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      80 µg
      Specification
      Macrophages
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Apr 12 2017

      What is the specificity of this ab towards Drosophila?

      DISCOUNT CODE: XXXXXXXXXX
      Expiration date: 03-04-2012
      Value: £227

      I am very pleased to hear you would like to accept our offer and test ab1398 in Drosophila. This code will give you £227 off your next order before the expir...

      Read More

      Hi,
      I'm potentially interested in buying an anti-GABARAP antibody for use in immunofluorescent staining. I have found that the sequence of Drosophila melanogaster Atg8a is identical in some regions to that of human GABARAP - are any of your anti-G...

      Read More

      Thank you very much for your interest in our antibodies.

      According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and mor...

      Read More

      I acquired the antibody ab1398 generated against a synthetic peptide, but I would like to know against which specific sequence was generated. Thank you very much in advance

      Thank you for your enquiry. The immunogen sequence used corresponds to: amino acids 15-31 (RSEGEKIRKKYPDRVPV) of the GABARAP protein. the data sheet has been updated to reflect this information. If you need any further help please do not hesitate to ge...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up