Key features and details
- Rabbit polyclonal to GABARAP
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab1398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000.|
FunctionUbiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Tissue specificityHeart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the ATG8 family.
modificationsThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
Cellular localizationEndomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
- Information by UniProt
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
Anti-GABARAP antibody (ab1398) at 1 µg/ml + Mouse macrophage whole cell lysate at 80 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ab1398 has been referenced in 3 publications.
- Li L et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . PubMed: 28534953
- Ning HJ et al. Propofol reduces hypoxia-induced autophagic cell death through downregulating HIF 1a in alveolar epithelial type II cells of rats. Mol Med Rep 16:1509-1515 (2017). PubMed: 28586054
- Belichenko PV et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . PubMed: 19034952