Anti-GABARAP antibody (ab1398)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-GABARAP antibody
See all GABARAP primary antibodies -
DescriptionRabbit polyclonal to GABARAP
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Host speciesRabbit
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SpecificityThis antibody recognizes human GABARAP (14 kD).
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide:
RSEGEKIRKKYPDRVPV
, corresponding to amino acids 15-31 of Human GABARAP.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading...
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PurityAffinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab1398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | 1/1000 - 1/5000. | |
WB | 1/500 - 1/1000. |
Target
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FunctionUbiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
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Tissue specificityHeart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
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Sequence similaritiesBelongs to the ATG8 family.
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Post-translational
modificationsThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
Cellular localizationEndomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
- Information by UniProt
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Database links
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Omim: 605125 Human
- SwissProt: O95166 Human
- SwissProt: Q9DCD6 Mouse
- Unigene: 647421 Human
- Unigene: 272460 Mouse
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Alternative names
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
Images
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Anti-GABARAP antibody (ab1398) at 1 µg/ml + Macrophage whole cell lysate at 80 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li L et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
- Belichenko PV et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . Read more (PubMed: 19034952) »