Anti-GABARAP antibody (ab1398)

Reviews (1)Q&A (3)References (2)

Overview

  • Product name
    Anti-GABARAP antibody
    See all GABARAP primary antibodies
  • Description
    Rabbit polyclonal to GABARAP
  • Host species
    Rabbit
  • Specificity
    This antibody recognizes human GABARAP (14 kD).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    RSEGEKIRKKYPDRVPV

    , corresponding to amino acids 15-31 of Human GABARAP.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab1398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Western Blot: use at 1:500-1:1,000 dilution.
    ELISA: use at 1:1,000-1:5,000 dilution

    • Target

    • Function
      Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    • Tissue specificity
      Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the ATG8 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
    • Cellular localization
      Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
    • Information by UniProt
    • Database links
      • Alternative names
        • ATG8A antibody
        • FLC 3B antibody
        • FLC3B antibody
        • FLJ25768 antibody
        • GABA type A receptor associated protein antibody
        • GABA(A) receptor associated protein antibody
        • GABA(A) receptor-associated protein antibody
        • GABARAP a antibody
        • GABARAP antibody
        • Gamma aminobutyric acid receptor associated protein antibody
        • Gamma-aminobutyric acid receptor-associated protein antibody
        • GBRAP_HUMAN antibody
        • MGC120154 antibody
        • MGC120155 antibody
        • MM 46 antibody
        • MM46 antibody
        see all

      Images

      References

      This product has been referenced in:
      • Li L  et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . Read more (PubMed: 28534953) »
      • Belichenko PV  et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . Read more (PubMed: 19034952) »
      See all 2 Publications for this product

      Publishing research using ab1398? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-GABARAP antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Macrophages)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      80 µg
      Specification
      Macrophages
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 12 2017

      Question

      What is the specificity of this ab towards Drosophila?

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      Answer

      DISCOUNT CODE: XXXXXXXXXX
      Expiration date: 03-04-2012
      Value: £227

      I am very pleased to hear you would like to accept our offer and test ab1398 in Drosophila. This code will give you £227 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab1398 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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      Question

      Hi,
      I'm potentially interested in buying an anti-GABARAP antibody for use in immunofluorescent staining. I have found that the sequence of Drosophila melanogaster Atg8a is identical in some regions to that of human GABARAP - are any of your anti-GABARAP antibodies raised against peptides idenitical to sequences contained within the Atg8a? I can see that ab1398 is raised against a region where there are a few differences, would you be able to let me know which regions the other 4 GABARAP antibodies are raised against/whether they are raised against a region identical to one found in Atg8a? I have attached a sequence alignment of human GABARAP (CAG47031.1) vs Drosophila Atg8a (NP_727447.1).
      Many thanks,

      Read More
      Answer

      Thank you very much for your interest in our antibodies.

      According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and more.

      I checked our other Anti-GABARAP antibodies and would like to recommend ab109364 (https://www.abcam.com/index.html?datasheet=109364 (or use the following: https://www.abcam.com/index.html?datasheet=109364).). The immunogen shows 92% homology with Drosophila melanogaster and therefore it is likely to cross react.

      To our knowledge, ab109364 has not been experimentally tested in Drosophila melanogaster. Therefore, I can offer a discount off a future purchase if you buy ab109364 now, test it in Drosophila melanogaster and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab109364 off a future order.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab109364 in Drosophila melanogaster. I will then send a discount code. This code must be issued before purchasing ab109364 so please wait for my reply before ordering.

      2. Purchase ab109364 either by phone, fax, or online (www.abcam.com).

      3. Test it in Drosophila melanogaster.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab109364 turns out to be unsuitable for Drosophila melanogaster, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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      Question

      I acquired the antibody ab1398 generated against a synthetic peptide, but I would like to know against which specific sequence was generated. Thank you very much in advance

      Read More
      Answer

      Thank you for your enquiry. The immunogen sequence used corresponds to: amino acids 15-31 (RSEGEKIRKKYPDRVPV) of the GABARAP protein. the data sheet has been updated to reflect this information. If you need any further help please do not hesitate to get back in touch with us.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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