Overview

  • Product name

  • Description

    Rabbit polyclonal to GABARAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human GABARAP (C terminal).
    Database link: O95166

  • Positive control

    • WB: HEK-293T whole cell lysate; Mouse kidney lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 14-16 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/100.

Target

  • Function

    Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificity

    Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the ATG8 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
  • Cellular localization

    Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG8A antibody
    • FLC 3B antibody
    • FLC3B antibody
    • FLJ25768 antibody
    • GABA type A receptor associated protein antibody
    • GABA(A) receptor associated protein antibody
    • GABA(A) receptor-associated protein antibody
    • GABARAP a antibody
    • GABARAP antibody
    • Gamma aminobutyric acid receptor associated protein antibody
    • Gamma-aminobutyric acid receptor-associated protein antibody
    • GBRAP_HUMAN antibody
    • MGC120154 antibody
    • MGC120155 antibody
    • MM 46 antibody
    • MM46 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for GABARAP (green) using ab223569 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223569 (D) Merge.

     

  • Anti-GABARAP antibody (ab223569) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13.9,40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    Blocking buffer: 5% milk in TBST.

    Color Development: TMB solution.

    Primary and secondary antibodies were incubated for 1 hour at RT.

  • Anti-GABARAP antibody (ab223569) at 1/1000 dilution + Mouse kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa
    Observed band size: 13.9,40 kDa why is the actual band size different from the predicted?


    Exposure time: 12 minutes


    Block: 5% milk + TBST

References

ab223569 has not yet been referenced specifically in any publications.

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