Anti-GABARAP antibody - N-terminal (ab227516)

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Overview

  • Product name

    Anti-GABARAP antibody - N-terminal
    See all GABARAP primary antibodies

  • Description

    Rabbit polyclonal to GABARAP - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Rhesus monkey

  • Immunogen

    Synthetic peptide within Human GABARAP (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: O95166

  • Positive control

    • WB: HEK-293T, U-87 MG, SK-N-SH, IMR32 and SH-N-AS whole cell extracts. ICC/IF: U-87 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

  • Tissue specificity

    Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the ATG8 family.

  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.

  • Cellular localization

    Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ATG8A antibody
    • FLC 3B antibody
    • FLC3B antibody
    • FLJ25768 antibody
    • GABA type A receptor associated protein antibody
    • GABA(A) receptor associated protein antibody
    • GABA(A) receptor-associated protein antibody
    • GABARAP a antibody
    • GABARAP antibody
    • Gamma aminobutyric acid receptor associated protein antibody
    • Gamma-aminobutyric acid receptor-associated protein antibody
    • GBRAP_HUMAN antibody
    • MGC120154 antibody
    • MGC120155 antibody
    • MM 46 antibody
    • MM46 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)
    Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)

    U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for GABARAP (green) using ab227516 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T transfected with GABARAP shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    15% SDS-PAGE

  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    15% SDS-PAGE

References

ab227516 has not yet been referenced specifically in any publications.

Publishing research using ab227516? Please let us know so that we can cite the reference in this datasheet.

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