Anti-GABARAP antibody - N-terminal (ab227516)
Key features and details
- Rabbit polyclonal to GABARAP - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAP antibody - N-terminal
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Rhesus monkey
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Immunogen
Synthetic peptide within Human GABARAP (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: O95166 -
Positive control
- WB: HEK-293T, U-87 MG, SK-N-SH, IMR32 and SH-N-AS whole cell extracts. ICC/IF: U-87 MG cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 14 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. -
Tissue specificity
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the ATG8 family. -
Post-translational
modificationsThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
Cellular localization
Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 327715 Cow
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Entrez Gene: 100462752 Pig
- Entrez Gene: 100008883 Rabbit
- Entrez Gene: 58974 Rat
- Entrez Gene: 714597 Rhesus monkey
- Omim: 605125 Human
see all -
Alternative names
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)
U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for GABARAP (green) using ab227516 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Western blot - Anti-GABARAP antibody - N-terminal (ab227516)All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T transfected with GABARAP shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
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Western blot - Anti-GABARAP antibody - N-terminal (ab227516)All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227516 has not yet been referenced specifically in any publications.
