For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    gabarap-antibody-n-terminal-ab227516.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Intracellular Signaling Adapters
Share by email

Anti-GABARAP antibody - N-terminal (ab227516)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)
  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)

Key features and details

  • Rabbit polyclonal to GABARAP - N-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human GABARAP protein (Fc Chimera) (ab191929)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GABARAP antibody - N-terminal
    See all GABARAP primary antibodies
  • Description

    Rabbit polyclonal to GABARAP - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human GABARAP (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: O95166

  • Positive control

    • WB: HEK-293T, U-87 MG, SK-N-SH, IMR32 and SH-N-AS whole cell extracts. ICC/IF: U-87 MG cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Adapters
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • GABA Receptors
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • SK N SH whole cell lysate (ab3956)
  • Recombinant Protein

    • Recombinant Human GABARAP protein (Fc Chimera) (ab191929)
  • Related Products

    • Recombinant Human GABARAP protein (ab151871)
    • Recombinant Human GABARAP protein (Fc Chimera) (ab191929)

Applications

Our Abpromise guarantee covers the use of ab227516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificity

    Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the ATG8 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
  • Cellular localization

    Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  • Target information above from: UniProt accession O95166 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 327715 Cow
    • Entrez Gene: 11337 Human
    • Entrez Gene: 56486 Mouse
    • Entrez Gene: 100462752 Pig
    • Entrez Gene: 100008883 Rabbit
    • Entrez Gene: 58974 Rat
    • Entrez Gene: 714597 Rhesus monkey
    • Omim: 605125 Human
    • SwissProt: Q9GJW7 Cow
    • SwissProt: O95166 Human
    • SwissProt: Q9DCD6 Mouse
    • SwissProt: Q8MK68 Rabbit
    • SwissProt: P60517 Rat
    • Unigene: 647421 Human
    • Unigene: 272460 Mouse
    • Unigene: 8411 Rat
    see all
  • Alternative names

    • ATG8A antibody
    • FLC 3B antibody
    • FLC3B antibody
    • FLJ25768 antibody
    • GABA type A receptor associated protein antibody
    • GABA(A) receptor associated protein antibody
    • GABA(A) receptor-associated protein antibody
    • GABARAP a antibody
    • GABARAP antibody
    • Gamma aminobutyric acid receptor associated protein antibody
    • Gamma-aminobutyric acid receptor-associated protein antibody
    • GBRAP_HUMAN antibody
    • MGC120154 antibody
    • MGC120155 antibody
    • MM 46 antibody
    • MM46 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)
    Immunocytochemistry/ Immunofluorescence - Anti-GABARAP antibody - N-terminal (ab227516)

    U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for GABARAP (green) using ab227516 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T transfected with GABARAP shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    15% SDS-PAGE

  • Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    Western blot - Anti-GABARAP antibody - N-terminal (ab227516)
    All lanes : Anti-GABARAP antibody - N-terminal (ab227516) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human brain neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    15% SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227516? Please let us know so that we can cite the reference in this datasheet.

    ab227516 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab227516.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.