Overview

  • Product name

    Anti-GABARAP antibody - N-terminal
    See all GABARAP primary antibodies
  • Description

    Rabbit polyclonal to GABARAP - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Human GABARAP aa 11-21 (N terminal) (Cysteine residue).
    Sequence:

    FEKRRSEGEKI-C


    Database link: O95166

  • Positive control

    • WB: HEK-293 and Odora cell lysates. ICC/IF: Human white blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
WB 1/200 - 1/1000. Predicted molecular weight: 13 kDa.

Target

  • Function

    Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificity

    Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the ATG8 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
  • Cellular localization

    Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG8A antibody
    • FLC 3B antibody
    • FLC3B antibody
    • FLJ25768 antibody
    • GABA type A receptor associated protein antibody
    • GABA(A) receptor associated protein antibody
    • GABA(A) receptor-associated protein antibody
    • GABARAP a antibody
    • GABARAP antibody
    • Gamma aminobutyric acid receptor associated protein antibody
    • Gamma-aminobutyric acid receptor-associated protein antibody
    • GBRAP_HUMAN antibody
    • MGC120154 antibody
    • MGC120155 antibody
    • MM 46 antibody
    • MM46 antibody
    see all

Images

  • Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.

    MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).

    The merged image is shown.

  • Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Developed using the ECL technique.

    Predicted band size: 13 kDa

  • Anti-GABARAP antibody - N-terminal (ab228504) at 1/100 dilution + Odora (human olfactory cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 13 kDa

  • Human white blood cells stained for GABARAP (red) using ab228504 incubated for 1 hour at room temperature at 1/200 dilution in ICC/IF.

    MHC class II staining is shown (green). Nuclear counterstain is Hoechst Dye (blue).

    The merged image is shown in panel C.

     

  • Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.

  • Human white blood cells stained for GABARAP (red) using ab228504 at 1/200 dilution in ICC/IF.

    MHC class staining is shown (green). The nuclear counterstain is Hoechst Dye (blue).

    The merged image is shown.

References

ab228504 has not yet been referenced specifically in any publications.

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