Overview

  • Product name

  • Description

    Rabbit polyclonal to GABARAPL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human GABARAPL1.
    Database link: Q9H0R8

  • Positive control

    • WB: HEK-293T and HeLa whole cell lysates. ICC/IF: HeLa cells (+/- treatment with Chloroquine).

Properties

Applications

Our Abpromise guarantee covers the use of ab229729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificity

    Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • Sequence similarities

    Belongs to the ATG8 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Images

  • All lanes : Anti-GABARAPL1 antibody (ab229729) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-GABARAPL1 antibody (ab229729) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : GABARAPL1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 14 kDa



    15% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells untreated (left panel) and treated with 50 μM Chloroquine for 24 hours (right panel) stained for GABARAPL1 (green) using ab229729  at 1/1000 dilution in ICC/IF.

    Red: Phalloidin, a F-actin marker.

References

ab229729 has not yet been referenced specifically in any publications.

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