Overview

  • Product name

    Anti-GABARAPL2/GATE-16 antibody [EP4808]
    See all GABARAPL2/GATE-16 primary antibodies
  • Description

    Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GABARAPL2/GATE-16 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, Human fetal brain, Human heart and Human spleen lysates.
  • General notes

    Previously labelled as GABARAPL2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.
    • Tissue specificity

      Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
    • Sequence similarities

      Belongs to the MAP1 LC3 family.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATG8 antibody
      • ATG8C antibody
      • FLC3A antibody
      • GABA(A) receptor-associated protein-like 2 antibody
      • Gabarapl2 antibody
      • Gamma-aminobutyric acid receptor-associated protein-like 2 antibody
      • Ganglioside expression factor 2 antibody
      • GATE-16 antibody
      • GATE16 antibody
      • GBRL2_HUMAN antibody
      • GEF-2 antibody
      • GEF2 antibody
      • General protein transport factor p16 antibody
      • Golgi-associated ATPase enhancer of 16 kDa antibody
      • MAP1 light chain 3-related protein antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: GABARAPL2 / GATE-16 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human tonsil lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab126607 was shown to specifically react with GABARAPL2 / GATE-16 when GABARAPL2 / GATE-16 knockout samples were used. Wild-type and GABARAPL2 / GATE-16 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Human heart tissue lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Agrotis A  et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). Read more (PubMed: 30661429) »
    • Habisov S  et al. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 291:9025-41 (2016). Read more (PubMed: 26929408) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1)
    Gel Running Conditions
    Reduced Denaturing (12,5)
    Loading amount
    20 µg
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 06 2016

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y cells)
    Gel Running Conditions
    Reduced Denaturing (13%)
    Loading amount
    30 µg
    Specification
    SH-SY5Y cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. George Allen

    Verified customer

    Submitted Sep 25 2013

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