Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4808] to GABARAPL2/GATE-16
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Product nameAnti-GABARAPL2/GATE-16 antibody [EP4808]
See all GABARAPL2/GATE-16 primary antibodies
DescriptionRabbit monoclonal [EP4808] to GABARAPL2/GATE-16
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GABARAPL2/GATE-16 aa 50-150. The exact sequence is proprietary.
- HeLa, Human fetal brain, Human heart and Human spleen lysates.
Previously labelled as GABARAPL2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab126607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
FunctionInvolved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.
Tissue specificityUbiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
Sequence similaritiesBelongs to the MAP1 LC3 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus.
- Information by UniProt
- ATG8 antibody
- ATG8C antibody
- FLC3A antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GABARAPL2 / GATE-16 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126607 was shown to specifically react with GABARAPL2 / GATE-16 when GABARAPL2 / GATE-16 knockout samples were used. Wild-type and GABARAPL2 / GATE-16 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-GABARAPL2/GATE-16 antibody [EP4808] (ab126607) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126607 has been referenced in 3 publications.
- Agrotis A et al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy 15:976-997 (2019). PubMed: 30661429
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Habisov S et al. Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation. J Biol Chem 291:9025-41 (2016). PubMed: 26929408