Anti-GABARAPL2/GATE-16 antibody - N-terminal (ab229525)
Key features and details
- Rabbit polyclonal to GABARAPL2/GATE-16 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GABARAPL2/GATE-16 antibody - N-terminal
See all GABARAPL2/GATE-16 primary antibodies -
Description
Rabbit polyclonal to GABARAPL2/GATE-16 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Synthetic peptide within Human GABARAPL2/GATE-16 (N terminal). The exact sequence is proprietary.
Database link: P60520 -
Positive control
- WB: Mouse and rat brain tissue extracts; SK-N-AS whole cell extract.
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General notes
Previously labelled as GABARAPL2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
Target
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Function
Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. -
Tissue specificity
Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 282531 Cow
- Entrez Gene: 11345 Human
- Entrez Gene: 93739 Mouse
- Entrez Gene: 100359937 Rat
- Entrez Gene: 64670 Rat
- Entrez Gene: 714413 Rhesus monkey
- Entrez Gene: 734814 Xenopus laevis
- Entrez Gene: 403036 Zebrafish
see all -
Alternative names
- ATG8 antibody
- ATG8C antibody
- FLC3A antibody
see all
Images
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Western blot - Anti-GABARAPL2/GATE-16 antibody - N-terminal (ab229525)All lanes : Anti-GABARAPL2/GATE-16 antibody - N-terminal (ab229525) at 1/1000 dilution
Lane 1 : Mouse brain tissue extracts at 15 µg
Lane 2 : SK-N-AS whole cell extracts at 40 µg
Lane 3 : Rat brain tissue extracts at 15 µg
Developed using the ECL technique.
Predicted band size: 14 kDa15% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229525 has not yet been referenced specifically in any publications.
