Product nameAnti-GABPA antibody
DescriptionRabbit polyclonal to GABPA
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- ICC/IF: U-251 MG cells. WB: RT4 and U-251 MG cell lysate; Human tonsil tissue lysate. IHC-P: Human lymph node tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||1/100 - 1/250. Predicted molecular weight: 51 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
- Information by UniProt
- E4TF 1A antibody
- E4TF1 60 antibody
- E4TF1A antibody
All lanes : Anti-GABPA antibody (ab224325) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3 : Human liver tissue lysate
Lane 4 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 51 kDa
Paraffin-embedded human lymph node tissue stained for GABPA using ab224325 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GABPA (green) using ab224325 at 4 µg/ml in ICC/IF.
ab224325 has not yet been referenced specifically in any publications.