Overview

  • Product name

    Anti-GABRB1 antibody [N96/55]
    See all GABRB1 primary antibodies
  • Description

    Mouse monoclonal [N96/55] to GABRB1
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against GABA A Receptor beta 2 or GABA A Receptor beta 3.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Mouse GABRB1 aa 327-450 (C terminal).
    Database link: P50571

  • Positive control

    • WB: rat brain lysate. IHC-P: Mouse backskin tissue. ICC/IF: HaCat cells.
  • General notes

    The clone number has been updated from S96-55 to N96/55, both clone numbers name the same antibody clone.

     

     This product was previously labelled as GABA A Receptor beta 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab93612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/100.

[ 0.1 - 1 µg/mL (Perox), 1 - 10 µg/mL (IF) ]

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW061132 antibody
    • B230208N19Rik antibody
    • GABA(A) receptor beta 1 antibody
    • GABA(A) receptor subunit beta-1 antibody
    • GABA-A receptor, beta-1 polypeptide antibody
    • Gabrb-1 antibody
    • GABRB1 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
    • Gamma Aminobutyric Acid A Receptor Beta 1 antibody
    • Gamma Aminobutyric Acid Receptor , beta-1 antibody
    • Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
    • Gamma-aminobutyric acid receptor subunit beta-1 antibody
    • GARB1 antibody
    • GBRB1_HUMAN antibody
    see all

Images

  • Western blot detection of GABRB1 in Rat brain membrane lysates using ab96612 at 1:1000 dilution.

  • Paraffin-embedded mouse backskin tissue fixed with Bouin's fixative, stained for GABRB1 using ab93612 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. FITC-conjugated goat anti-mouse (green) at 1/50 was used as the secondary antibody. Secondary was incubated at 1 hour at room temperature.

  • HaCaT (Human keratinocyte cell line) cells stained gor GABRB1 using ab93612 at 1/100 dilution in ICC/IF. Cells were fixed using 100% methanol for 10 minutes at -20°C and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was FITC-conjugated goat anti-mouse (green) at 1/50 incubated for 1 hour at room temperature.

References

This product has been referenced in:

  • Bates RC  et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). Read more (PubMed: 24836856) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.
Upon checking with the lab, they confirmed that any type of IHC has not yet been tested with this antibody so far. I apologize for any inconveniences caused.

Thus, if your colleagues had problems with this antibody in the past in IHC-P or IHC-Fr, I'd be happy to replace, credit or refund the antibody. Alternatively, I can give you a testing discount code for trying the antibody in IHC-P and IHC-Fr.

In case you would liketo proceed with the IHC experiments, I am sending you the general IHC protocol that has been used successfully with many antibodies. Please see the attached protocol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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