Key features and details
- Mouse monoclonal [N96/55] to GABRB1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-GABRB1 antibody [N96/55]
See all GABRB1 primary antibodies
DescriptionMouse monoclonal [N96/55] to GABRB1
SpecificityNo cross-reactivity against GABA A Receptor beta 2 or GABA A Receptor beta 3.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Mouse GABRB1 aa 327-450 (C terminal).
Database link: P50571
- WB: rat brain lysate. IHC-P: Mouse backskin tissue. ICC/IF: HaCat cells.
The clone number has been updated from S96-55 to N96/55, both clone numbers name the same antibody clone.
This product was previously labelled as GABA A Receptor beta 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).|
[ 0.1 - 1 µg/mL (Perox), 1 - 10 µg/mL (IF) ]
FunctionGABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- AW061132 antibody
- B230208N19Rik antibody
- GABA(A) receptor beta 1 antibody
Western blot detection of GABRB1 in Rat brain membrane lysates using ab96612 at 1:1000 dilution.
Paraffin-embedded mouse backskin tissue fixed with Bouin's fixative, stained for GABRB1 using ab93612 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. FITC-conjugated goat anti-mouse (green) at 1/50 was used as the secondary antibody. Secondary was incubated at 1 hour at room temperature.
HaCaT (Human keratinocyte cell line) cells stained gor GABRB1 using ab93612 at 1/100 dilution in ICC/IF. Cells were fixed using 100% methanol for 10 minutes at -20°C and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was FITC-conjugated goat anti-mouse (green) at 1/50 incubated for 1 hour at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93612 has been referenced in 1 publication.
- Bates RC et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). PubMed: 24836856