Anti-GABRB1 antibody [N96/55] (ab93612)

Submit a review Q&A (1)References (1)
Western blot - Anti-GABRB1 antibody [N96/55] (ab93612)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 antibody [N96/55] (ab93612)
  • Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 antibody [N96/55] (ab93612)

Key features and details

  • Mouse monoclonal [N96/55] to GABRB1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-GABRB1 antibody [N96/55]
    See all GABRB1 primary antibodies

  • Description

    Mouse monoclonal [N96/55] to GABRB1

  • Host species

    Mouse

  • Specificity

    No cross-reactivity against GABA A Receptor beta 2 or GABA A Receptor beta 3.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Fusion protein corresponding to Mouse GABRB1 aa 327-450 (C terminal).
    Database link: P50571

  • Positive control

    • WB: rat brain lysate. IHC-P: Mouse backskin tissue. ICC/IF: HaCat cells.

  • General notes

    The clone number has been updated from S96-55 to N96/55, both clone numbers name the same antibody clone.

     

     This product was previously labelled as GABA A Receptor beta 1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab93612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/100.

[ 0.1 - 1 µg/mL (Perox), 1 - 10 µg/mL (IF) ]

Target

  • Function

    GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.

  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AW061132 antibody
    • B230208N19Rik antibody
    • GABA(A) receptor beta 1 antibody
    • GABA(A) receptor subunit beta-1 antibody
    • GABA-A receptor, beta-1 polypeptide antibody
    • Gabrb-1 antibody
    • GABRB1 antibody
    • Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
    • Gamma Aminobutyric Acid A Receptor Beta 1 antibody
    • Gamma Aminobutyric Acid Receptor , beta-1 antibody
    • Gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 antibody
    • Gamma-aminobutyric acid receptor subunit beta-1 antibody
    • GARB1 antibody
    • GBRB1_HUMAN antibody
    see all

Images

  • Western blot - Anti-GABRB1 antibody [N96/55] (ab93612)
    Western blot - Anti-GABRB1 antibody [N96/55] (ab93612)

    Western blot detection of GABRB1 in Rat brain membrane lysates using ab96612 at 1:1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 antibody [N96/55] (ab93612)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 antibody [N96/55] (ab93612)

    Paraffin-embedded mouse backskin tissue fixed with Bouin's fixative, stained for GABRB1 using ab93612 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. FITC-conjugated goat anti-mouse (green) at 1/50 was used as the secondary antibody. Secondary was incubated at 1 hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 antibody [N96/55] (ab93612)
    Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 antibody [N96/55] (ab93612)

    HaCaT (Human keratinocyte cell line) cells stained gor GABRB1 using ab93612 at 1/100 dilution in ICC/IF. Cells were fixed using 100% methanol for 10 minutes at -20°C and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was FITC-conjugated goat anti-mouse (green) at 1/50 incubated for 1 hour at room temperature.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab93612? Please let us know so that we can cite the reference in this datasheet.

ab93612 has been referenced in 1 publication.

  • Bates RC  et al. Reduced CHRNA7 expression in C3H mice is associated with increases in hippocampal parvalbumin and glutamate decarboxylase-67 (GAD67) as well as altered levels of GABA(A) receptor subunits. Neuroscience 273:52-64 (2014). PubMed: 24836856

Customer reviews and Q&As

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Submit a question

Question

send protocol details for IHC-Fr and IHC-P

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Answer

Thank you for contacting us.
Upon checking with the lab, they confirmed that any type of IHC has not yet been tested with this antibody so far. I apologize for any inconveniences caused.

Thus, if your colleagues had problems with this antibody in the past in IHC-P or IHC-Fr, I'd be happy to replace, credit or refund the antibody. Alternatively, I can give you a testing discount code for trying the antibody in IHC-P and IHC-Fr.

In case you would liketo proceed with the IHC experiments, I am sending you the general IHC protocol that has been used successfully with many antibodies. Please see the attached protocol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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